Product Class: Other

Lambda Exonuclease
NEBU cloned at NEB recombinant 37 75 Heat

Product Introduction

  • DNA specific exonuclease
  • Catalyzes the removal of nucleotides from linear or nicked double-stranded DNA in the 5' to 3' direction
  • Highly processive degradation of double-stranded DNA from the 5' end
  • Preferred substrate is 5'-phosphorylated double-stranded DNA although non-phosphorylated substrates are degraded at a greatly reduced rate
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

Lambda Exonuclease is ideal for:

  • Conversion of linear double-stranded DNA to single-stranded DNA via preferred activity on 5'-phosphorylated ends
Catalog # Size Concentration
M0262S 1000.0 units 5000 units/ml
M0262L 5000.0 units 5000 units/ml

Product Information

Description

Highlights

Isolated from a recombinant source
Highly processive 5'→3' exonuclease
Supplied in 10X Reaction Buffer

Product Source

A genetic fusion of the E. coli Lambda Exonuclease gene with the gene encoding maltose binding protein (MBP). Following affinity chromatography, Lambda Exonuclease is cleaved from the fusion construct and purified away from MBP. 
This product is related to the following categories:

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • M0262S     -20    
      Lambda Exonuclease M0262SVIAL -20 1 x 0.2 ml 5,000 units/ml
      Lambda Exonuclease Reaction Buffer B0262SVIAL -20 1 x 1.5 ml 10 X
  • M0262L     -20    
      Lambda Exonuclease M0262LVIAL -20 1 x 1 ml 5,000 units/ml
      Lambda Exonuclease Reaction Buffer B0262SVIAL -20 1 x 1.5 ml 10 X

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X Lambda Exonuclease Reaction Buffer with 1 μg sonicated duplex [3H]-DNA.

Reaction Conditions

1X Lambda Exonuclease Reaction Buffer
Incubate at 37°C

1X Lambda Exonuclease Reaction Buffer
67 mM Glycine-KOH
2.5 mM MgCl2
50 µg/ml BSA
(pH 9.4 @ 25°C)

Usage Concentration

5,000 units/ml

Storage Buffer

25 mM Tris-HCl
50 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 8 @ 25°C

Heat Inactivation

75°C for 10 minutes

Molecular Weight

Theoretical: 28000 daltons

Specific Activity

50,000 units/mg

Product Notes

  1. The activity of Lambda Exonuclease in various buffers is listed below:

    • NEBuffer 1 - 15%
    • NEBuffer 2 - 40%
    • NEBuffer 3 -10%
    • NEBuffer 4 - 40%
    • CutSmart – 50%
    • Exonuclease I Reaction Buffer- 100%
    • Thermopol Reaction Buffer -100%
  2. 5´-OH ends are digested 20X slower than 5´-PO4 ends. ssDNA is digested 100X slower than dsDNA.   

References

  1. Little, J.W. (1981). Gene Amplif. Anal. 2, 135-145.

Protocols, Manuals & Usage

Protocols

  1. Protocol for Lambda Exonuclease (M0262)

Usage & Guidelines

Tools & Resources

Selection Charts

Web Tools

Research Posters

FAQs & Troubleshooting

FAQs

  1. What is the activity of M0262 Lambda Exonuclease in various NEB buffers?
  2. How Does T5 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
  3. How Does T7 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
  4. Will Lambda Exonuclease work in rCutSmart Buffer?
  5. Can Exonuclease I be used with a double stranded exonuclease to clean up plasmid preparations?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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