Product Class: Other

TaqI Methyltransferase
neb31 cloned at NEB recombinant 65 No SAM

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

M0219TaqI_thumb

Product Introduction

  • TaqI Methyltransferase modifies the adenine residue (N6) of the sequence TCGA
  • Supplied with 10X rCutSmart™ Buffer and 32 mM S-adenosylmethionine (SAM)
Catalog # Size Concentration
M0219S 1000.0 units 10000 units/ml

Product Information

Description

TaqI Methyltransferase modifies the adenine residue (N6) of the sequence TCGA.

Product Source

An E. coli strain that carries the cloned TaqI modification gene from Thermus aquaticus.
This product is related to the following categories:
Methyltransferases for Epigenetics,
Methyltransferases Products,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to protect 1 μg λ DNA in 1 hour at 65°C in a total reaction volume of 20 μl against cleavage by TaqI restriction endonuclease.

Reaction Conditions

1X rCutSmart™ Buffer
Supplement with 80 µM S-adenosylmethionine (SAM)
Incubate at 65°C

1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Control Protection Assay

Protection Assay Conditions:
TaqI Methyltransferase is incubated with 1 µg λ DNA in 20 µl 1X rCutSmart Buffer and 80 µM S-adenosylmethionine, for 1 hour at 65°C. The extent of protection by TaqI Methyltransferase is determined by the addition of 30 µl 1X rCutSmart Buffer and 10 units of TaqI restriction endonuclease. Incubation for 15 minutes at 65°C is followed by analysis on an agarose gel.

Storage Buffer

10 mM Tris-HCl
100 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

No

Molecular Weight

Theoretical: 47845 daltons

Product Notes

  1. TaqI Methyltransferase gives 25% activity at 37°C.

References

  1. Hoffman, J.L. (1986). Biochemistry. 25, 4444-4449.

Protocols, Manuals & Usage

Protocols

  1. Recommended Protocol for 3H labeling of DNA
  2. Recommended Protocol for Methylation of Genomic DNA

FAQs & Troubleshooting

FAQs

  1. Is S-adenosylmethionine (SAM) supplied with the Methyltransferase?
  2. What should be considered if the methylation is not going to completion?
  3. What is the activity of TaqI Methyltransferase at 37°C?
  4. What is the activity of TaqI Methyltransferase at 50°C?
  5. Does TaqI Methyltransferase require MgCl2?
  6. Does TaqI Methyltransferase require BSA?
  7. What is the activity of TaqI Methyltransferase in other buffers?
  8. What is the molecular weight of TaqI Methyltransferase?
  9. Is TaqI Methyltransferase used in any special techniques?
  10. Will any NEB methyltransferases (methylases) work on RNA?
  11. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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