HpaII Methyltransferase
Product information| Code | Name | Size | Quantity | Price | |
|---|---|---|---|---|---|
M0214S |
HpaII Methyltransferase |
100 units ( 4000 units/ml ) | - | Unavailable in your region |
HpaII Methyltransferase
We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.
HpaII Methyltransferase recognizes the same sequence as the MspI Methyltransferase, but modifies the internal cytosine residue (C5) of the sequence CCGG.
- Supplied with 10x rCutSmart™ Buffer and 32 mM S-adenosylmethionine (SAM)
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Product Information
Product Source
An E. coli strain that carries the cloned HpaII modification gene from Haemophilus parainfluenzae (ATCC 49669).- This product is related to the following categories:
- Methyltransferases for Epigenetics,
- Methyltransferases
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Reagents Supplied
The following reagents are supplied with this product:
NEB # Component Name Component # Stored at (°C) Amount Concentration -
M0214S -20 HpaII Methyltransferase M0214SVIAL -20 1 x 0.025 ml 4,000 units/ml rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X S-adenosylmethionine (SAM) B9003SVIAL -20 1 x 0.1 ml 32 mM
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Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to protect 1 μg λ DNA in 1 hour at 37°C in a total reaction volume of 10 μl against cleavage by HpaII restriction endonuclease.Reaction Conditions
1X rCutSmart™ Buffer
Supplement with 80 µM S-adenosylmethionine (SAM)
Incubate at 37°C1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)Control Protection Assay
HpaII Methyltransferase is incubated with 1 µg of λ DNA in 10 µl 1X rCutSmart Buffer, supplemented with 80 µM S-adenosylmethionine, for one hour at 37°C followed by 15 minutes at 65°C. The extent of protection by HpaII Methyltransferase is determined by the addition of 40 µl NEBuffer 1 supplemented with 10 mM MgCl2 and 10 units of HpaII restriction endonuclease. Incubation at 37°C for 30 minutes is followed by analysis on agarose gels.Storage Buffer
50 mM Tris-HCl
150 mM NaCl
0.1 mM EDTA
5 mM TCEP
200 µg/ml BSA
50% Glycerol
pH 7.5 @ 25°CHeat Inactivation
65°C for 20 minutesMolecular Weight
Theoretical: 40397 daltons
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Related Products
Materials Sold Separately
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References
- Hoffman, J.L. (1986). Biochemistry. 25, 4444-4449.
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Protocols, Manuals & Usage
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FAQs & Troubleshooting
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FAQs
- Is S-adenosylmethionine (SAM) supplied with the Methyltransferase?
- What should be considered if the methylation is not going to completion?
- Can HpaII Methyltransferase be heat inactivated?
- Does HpaII Methyltransferase require MgCl2?
- What is the molecular weight of HpaII Methyltransferase?
- Can HpaII methylated DNA be used to transform E. coli?
- Will any NEB methyltransferases (methylases) work on RNA?
- Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
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Citations & Technical Literature
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Citations
Product Citation Tool
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Quality, Safety & Legal
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Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here. -
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version] -
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- M0214S_L_v1_0181609
- M0214S_L_v1_0181703
- M0214S_L_v1_0181706
- M0214S_L_v1_0181709
- M0214S_L_v1_0181802
- M0214S_v2_10009371
- M0214S_v2_10022347
- M0214S_v2_10033756
- M0214S_v2_10036572
- M0214S_v2_10050229
- M0214S_v2_10056016
- M0214S_v2_10068166
- M0214S_v2_10083533
- M0214S_v2_10090418
- M0214S_v2_10095212
- M0214S_v2_10109221
- M0214S_v2_10137976
- M0214S_v2_10168971
- M0214S_v2_10207553
- M0214S_v2_10226329
- M0214S_v2_10235137
- M0214S_v2_10246040
- M0214S_v2_10262910
- M0214S_v2_10269454
- M0214S_v2_10291482
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Safety Data Sheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely. -
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
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