Product Class: Other

NEBNext® Circularization Module for MGI®

Product Introduction

The NEBNext Circularization Module for MGI is optimized for the circularization of double-stranded DNA MGI-compatible libraries to create single-stranded circular DNA libraries for sequencing on the MGI platform.

  • Fast workflow
  • High yields

Request a sample of this product by visiting the NEBNext Sample Request Page and choosing “Sample not listed” at the bottom of the list; your local NEB Account Manager will reach out to confirm the details of your request.

 

Catalog # Size Concentration
E9720S 24.0 reactions

Product Information

Description

The NEBNext Circularization Module for MGI provides reagents and a fast and streamlined workflow for the creation of single-stranded circular DNA libraries compatible with MGI sequencing.

The kit uses a splint ligation-based protocol to circularize MGI-compatible double-stranded DNA libraries produced using the NEBNext FS DNA Library Prep Kit for MGI (NEB #9705) and the NEBNext RNA Library Prep Kit for MGI (NEB #9710), in conjunction with the NEBNext Multiplex Oligos for MGI (Dual Index primer Pairs Set 1) (NEB #E9725).
This product is related to the following categories:
Library Preparation for MGI® Products,
Next Generation Sequencing Library Preparation Products,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • E9720S     -20    
      NEBNext® Circularization Reaction Buffer E9623AVIAL -20 1 x 0.12 ml Not Applicable
      NEBNext® Circularization Ligase E9624AVIAL -20 1 x 0.048 ml Not Applicable
      NEBNext® Splint Oligo for MGI / Complete Genomics E9626AVIAL -20 1 x 0.072 ml Not Applicable
      NEBNext® Exo Mix for MGI / Complete Genomics E9627AVIAL -20 1 x 0.048 ml Not Applicable
      TE Buffer (1X) E9614AVIAL -20 1 x 1.5 ml Not Applicable

Properties & Usage

Protocols, Manuals & Usage

Protocols

  1. Where can I find protocols for the  NEBNext® Circularization Module for MGI® (NEB #E9720)?

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

FAQs & Troubleshooting

FAQs

  1. How much library is required for the circularization reaction when using the NEBNext® Circularization Module for MGI® (NEB #E9635)?
  2. What solution should I use for diluting libraries or library pools to achieve 25 nM in 40 µl for the circularization reaction when using the NEBNext® Circularization Module for MGI® (NEB #E9720)?
  3. Are there safe stopping points in the circularization workflow when using the NEBNext® Circularization Module for MGI® (NEB #E9720)?
  4. Can I use the NEBNext® Circularization Module for MGI® (NEB #E9720) on libraries generated using reagents from other vendors?
  5. How long can the circles made using the NEBNext® Circularization Module for MGI® (NEB #E9720) be stored?
  6. Can libraries be pooled before the circularization reaction when using the NEBNext® Circularization Module for MGI® (NEB #E9720)?
  7. How do we proceed into sequencing after the circularization reaction (using the NEBNext® Circularization Module for MGI® (NEB #E97290))?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.