The Ph.D.-12™ Phage Display Peptide Library contains a single tube of the Ph.D. -12 Phage Library. The Ph.D.-12 library is a combinatorial library of random 12-mer peptides fused to a minor coat protein (pIII) of M13 phage. The displayed peptide is expressed at the N-terminus of pIII. The library consists of approximately 109 electroporated (i.e., unique) sequences.
Ready to use complex phage library
Applications and methodologies are numerous and supported by over 20 years of literature citations
Does not require helper phage for amplification
Inherent link between phenotype and genotype allows screening of billions of clones in a single microtiter well or Eppendorf tube
Product Information
The Ph.D.-12 Phage Display Peptide Library is based on a combinatorial library of random dodecapeptides fused to a minor coat protein (pIII) of M13 phage (1–6). The displayed peptide (12-mer) is expressed at the N-terminus of pIII, i.e., the first residue of the mature protein is the first randomized position. The peptide is followed by a short spacer (Gly-Gly-Gly-Ser) and then the wild-type pIII sequence. The library consists of approximately 109 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.
Giraldo A.MV., Mary C., Sivanesan D., Baron C. (2015) VirB6 and VirB10 from the Brucella type IV secretion system interact via the N-terminal periplasmic domain of VirB6 FEBS Lett; 589, 1883-9. PubMedID: 26071378, DOI: 10.1016/j.febslet.2015.05.051
Wang H., Fangxiao J., Liang H., Gu H. (2015) A Proline-Rich Domain in the Genotype 4 Hepatitis E Virus ORF3 PLoS One; 10, e0133282. PubMedID: 26177202, DOI: 10.1371/journal.pone.0133282
Zhang L., Zhang H., Fan Z., Zhou X., Yu L., Sun H., Wu Z., Yu Y., Song B., Ma J., Tong C., Wang X., Zhu Z., Cui Y. (2015) Identification of a Conserved Linear B-Cell Epitope of Streptococcus dysgalactiae GapC Protein by Screening Phage-Displayed Random Peptide Library PLoS One; 10, e0131221. PubMedID: 26121648, DOI: 10.1371/journal.pone.0131221
Maneewatch S, Adisakwattana P, Chaisri U, Saengjaruk P, Srimanote P, Thanongsaksrikul J, Sakolvaree Y, Poungpan P, Chaicumpa W (2014) Therapeutic epitopes of Leptospira LipL32 protein and their characteristics Protein Eng Des Sel; 27(5), 135-44. PubMedID: 24760832, DOI: 10.1093/protein/gzu006
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The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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