Product Class: Kit

Ph.D.™-12 Phage Display Peptide Library

Product Introduction

The Ph.D.-12™ Phage Display Peptide Library contains a single tube of the Ph.D. -12 Phage Library. The Ph.D.-12 library is a combinatorial library of random 12-mer peptides fused to a minor coat protein (pIII) of M13 phage. The displayed peptide is expressed at the N-terminus of pIII. The library consists of approximately 109 electroporated (i.e., unique) sequences.

  • Ready to use complex phage library
  • Applications and methodologies are numerous and supported by over 20 years of literature citations
  • Does not require helper phage for amplification
  • Inherent link between phenotype and genotype allows screening of billions of clones in a single microtiter well or Eppendorf tube
Catalog # Size Concentration
E8111L 50.0 panning experiments

Product Information

Description

The Ph.D.-12 Phage Display Peptide Library is based on a combinatorial library of random dodecapeptides fused to a minor coat protein (pIII) of M13 phage (1–6). The displayed peptide (12-mer) is expressed at the N-terminus of pIII, i.e., the first residue of the mature protein is the first randomized position. The peptide is followed by a short spacer (Gly-Gly-Gly-Ser) and then the wild-type pIII sequence. The library consists of approximately 109 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.

Citations for Phage Display



Panning with a pentavalent peptide library displayed on pIII.
This product is related to the following categories:
Phage Display Products
This product can be used in the following applications:
Phage Display

Kit Components

Kit Components

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

References

  1. Devlin, J.J., Panganiban, L.C. and Devlin, P.E. (1990). Science. 249, 404-406.
  2. Smith, G.P. and Scott, J.K. (1993). Methods Enzymol.. 217, 228-257. San Diego: Academic Press.
  3. Cortese et al. (1995). Curr. Opin. Biotechnol.. 6,
  4. Scott, J.K. and Smith, G.P. (1990). Science. 249, 386-390.
  5. Cwirla, S.E., Peters, E.A., Barret, R.W. and Dower, W.J. (1990). Proc. Natl. Acad. Sci. USA. 87, 6378-6382.
  6. Devlin, J.J., Panganiban, L.C. and Devlin, P.E. (1990). Science. 249, 404-406.

Protocols, Manuals & Usage

Protocols

  1. M13 Titer Protocol for Ph.D. Phage Display
  2. M13 Amplifcation Protocol for Ph.D. Phage Display

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

FAQs & Troubleshooting

FAQs

  1. Which of the three ready-made libraries should I choose?
  2. What peptide libraries are available for use with Ph.D.™ Phage Display?
  3. I am using Ph.D.™ Phage display and the amplified phage titer is low.
  4. I am using Ph.D.™ Phage Display and the phage DNA templates do not yield a readable sequence.
  5. I am using Ph.D.™ Phage Display and the sequencing templates do not run where they should on a gel.
  6. I am using Ph.D.™ Phage Display and after 4 or more rounds of panning all clones are wild-type phage (white plaques).
  7. When performing an experiment using Ph.D.™ Phage Display, the ELISA indicates that background binding to the plate is as high as binding to the target.
  8. When using the Ph.D.™ Phage Display, panning yielded a consensus sequence, but no ELISA signal.
  9. I am using Ph.D.™ Phage Display and the streptavidin control experiment did not yield the HPQ consensus sequence.
  10. Can a different bacterial strain be used with the Ph.D.™ Phage Display?
  11. Where can I find references for Ph.D.™ phage display libraries?
  12. What is the pIII sequence for an insertless clone? What is at the N-terminus of a PhD clone?
  13. How are the PhD Phage Display Libraries cloned?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Other Products You May Be Interested In