Product Class: Kit

NEBNext® Ultra™ II FS DNA Module

Product Introduction

This module is part of the NEBNext® Ultra™ II FS workflow, and is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595), for Illumina®-compatible library construction.

Kits that include reagents for every step in the Ultra II FS DNA library construction workflow are also available (NEBNext Ultra II FS DNA Library Prep Kit for Illumina NEB #E7805 and NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads NEB #E6177).

Note: The Ultra II FS (Fragmentation System) enzyme mix does not include NEBNext dsDNA Fragmentase®.

Catalog # Size Concentration
E7810S 24 reactions
E7810L 96 reactions

Product Information

Description




The NEBNext Ultra II FS DNA Module has been optimized to convert 100 pg - 0.5 μg of intact DNA to fragmented, end-repaired DNA having 5´ phosphorylated, 3´ dA-tailed ends. The module is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595), and is part of the Ultra II FS DNA workflow.

For Illumina library construction, the NEBNext Ultra II FS DNA Module is designed for use with the following:

For use with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1) (NEB #E7395), refer to the Protocols tab for UMI Adaptors-specific guidance. 

This product is related to the following categories:
DNA Fragmentation & RNA Fragmentation,
Automation for NEBNext® NGS Library Prep,
Next Generation Sequencing Library Preparation,

Kit Components

Kit Components

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • E7810S     -20    
      NEBNext® Ultra™ II FS Enzyme Mix E7806AVIAL -20 1 x 0.048 ml Not Applicable
      NEBNext® Ultra™ II FS Reaction Buffer E7807AVIAL -20 1 x 0.168 ml Not Applicable
      TE Buffer (1X) E7808AVIAL -20 1 x 1.1 ml 1 X
  • E7810L     -20    
      NEBNext® Ultra™ II FS Enzyme Mix E7806AAVIAL -20 1 x 0.192 ml Not Applicable
      NEBNext® Ultra™ II FS Reaction Buffer E7807AAVIAL -20 1 x 0.672 ml Not Applicable
      TE Buffer (1X) E7808AAVIAL -20 1 x 4.3 ml 1 X

Properties & Usage

Materials Required but not Supplied

Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)

Protocols, Manuals & Usage

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

FAQs & Troubleshooting

FAQs

  1. When preparing samples using the NEBNext Ultra II FS DNA Library Prep Kit, can my input DNA be in EDTA-containing solutions?
  2. What do I do if I see a precipitate in the Ultra II FS Reaction Buffer?
  3. Following the Fragmentation and End Prep steps of the NEBNext Ultra II FS workflow can the reactions be kept at 4° C?
  4. Do I really need to vortex the Ultra II FS Enzyme Mix?
  5. What is the concentration of my index primers?
  6. Which NEBNext ligation module should be used in conjunction with the NEBNext Ultra II FS DNA Module?
  7. Can I use the NEBNext Ultra II FS DNA Module for bisulfite conversion and EMSeq workflows?
  8. Does the fragmentation reagent included in NEBNext Ultra® II FS DNA kits contain NEBNext dsDNA Fragmentase®?
  9. Is the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® compatible with all sample types?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]


Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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