NEBNext® Ultra™ II FS DNA Module
Product information| Code | Name | Size | Quantity | Price | |
|---|---|---|---|---|---|
E7810S |
NEBNext Ultra II FS DNA Module |
24 rxns | - | Unavailable in your region | |
E7810L |
NEBNext Ultra II FS DNA Module |
96 rxns | - | Unavailable in your region |
NEBNext® Ultra™ II FS DNA Module
This module is part of the NEBNext® Ultra™ II FS workflow, and is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595), for Illumina®-compatible library construction.
Kits that include reagents for every step in the Ultra II FS DNA library construction workflow are also available (NEBNext Ultra II FS DNA Library Prep Kit for Illumina NEB #E7805 and NEBNext Ultra II FS DNA Library Prep with Sample Purification Beads NEB #E6177).
Note: The Ultra II FS (Fragmentation System) enzyme mix does not include NEBNext dsDNA Fragmentase®.
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Product Information
The NEBNext Ultra II FS DNA Module has been optimized to convert 100 pg - 0.5 μg of intact DNA to fragmented, end-repaired DNA having 5´ phosphorylated, 3´ dA-tailed ends. The module is optimized for use with the NEBNext Ultra II Ligation Module (NEB #E7595), and is part of the Ultra II FS DNA workflow.
For Illumina library construction, the NEBNext Ultra II FS DNA Module is designed for use with the following:
- NEBNext Ultra II Ligation Module (NEB #E7595)
- NEBNext Ultra II Q5® Master Mix (NEB #M0544)
- NEBNext Oligos for Illumina (NEB #E7335, #E7500, #E7710, #E7730, #E6609, #E7600 #E7535, #E7350)
For use with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1) (NEB #E7395), refer to the Protocols tab for UMI Adaptors-specific guidance.
- This product is related to the following categories:
- DNA Fragmentation & RNA Fragmentation,
- Automation for NEBNext® NGS Library Prep,
- Next Generation Sequencing Library Preparation
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Kit Components
The following reagents are supplied with this product:
NEB # Component Name Component # Stored at (°C) Amount Concentration -
Properties & Usage
Materials Required but not Supplied
Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)
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Protocols, Manuals & Usage
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Manuals
The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.
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FAQs & Troubleshooting
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FAQs
- When preparing samples using the NEBNext Ultra II FS DNA Library Prep Kit can my input DNA be in EDTA-containing solutions
- What do I do if I see a precipitate in the Ultra II FS Reaction Buffer
- Following the Fragmentation and End Prep steps of the NEBNext Ultra II FS workflow can the reactions be kept at 4C
- Do I really need to vortex the Ultra II FS Enzyme Mix
- What is the concentration of my index primers
- Which NEBNext ligation module should be used in conjunction with the NEBNext Ultra II FS DNA Module
- Can I use the NEBNext Ultra II FS DNA Module for bisulfite conversion and EMSeq workflows?
- Does the fragmentation reagent included in NEBNext Ultra® II FS DNA kits contain NEBNext dsDNA Fragmentase®?
- Is the NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina® compatible with all sample types?
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Citations & Technical Literature
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Citations
NEBNext Product Citations Search Tool
Use this tool to find citations related to NEBNext products. Search by product, keyword or instrument name – use the filters to select specific product areas.
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Feature Articles
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Quality, Safety & Legal
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Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here. -
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version] -
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number] -
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
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The supporting documents available for this product can be downloaded below.