Product Class: Kit

NEBNext® Fast DNA Library Prep Set for Ion Torrent™

The S size of this product has been discontinued as of September 2018. The L size will still be available.

Product Introduction

The NEBNext® Fast DNA DNA Library Prep Set for Ion Torrent® enables library preparation from pre-sheared DNA, with a streamlined, high-performance library prep workflow.

  • High yield 
  • Fast, simple workflow – under 2 hours, with minimal hands-on time 
  • Compatible with broad range of sample input (10 ng –1 μg)

If enzymatic shearing of DNA is preferred, a version of the kit that includes enzymatic fragmentation reagents is available.

Catalog # Size Concentration
E6270L 50 reactions

Product Information

Description



The NEBNext Fast DNA Library Prep Set for Ion Torrent is suitable when mechanical methods, such as Covaris® acoustic shearing, are preferred for fragmentation of input DNA. The components and protocol for this kit have been optimized for the volumes and concentrations of DNA sheared by mechanical methods, and the kit is compatible with input amounts from 10 ng - 1µg, with a workflow time of under 2 hours and ~12 min. hands-on time..
DNA Library Preparation Workflow for Ion TorrentDNA Library Preparation Workflow for Ion Torrent

Representative Results from Libraries Constructed Using NEBNext for Ion Torrent 
Representative Results from Libraries Constructed Using NEBNext for Ion Torrent
0.5 µg of DNA from 3 different genomes with varying GC content were used to construct 200 bp and 400 bp libraries using the NEBNext Fast DNA Fragmentation and Library Prep Set for Ion Torrent, analyzed by the Agilent® Bioanalyzer®.
Representative Ion Torrent Run Report
Representative Ion Torrent Run Report
A typical Ion Torrent run report for libraries made from E. coli (K12 MG1655 strain) genomic DNA using NEBNext Fast DNA Fragmentation & Library Prep Set for Ion Torrent. A. Alignment Summary. 72 Mb of data was generated, with an average genome coverage of 15.5 X, from approximately 0.5 Million 200 bp reads. B. Read length histogram from 200 bp run.

Lot Control

The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
This product is related to the following categories:
Library Preparation for Ion Torrent
This product can be used in the following applications:
DNA Sequencing

Kit Components

Kit Components

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • E6270L     -20    
      NEBNext® DNA Library Adaptors for Ion Torrent™ E6274AAVIAL -20 1 x 1 ml Not Applicable
      NEBNext End Repair Reaction Buffer E6272AAVIAL -20 1 x 0.3 ml 10 X
      T4 DNA Ligase E6275AAVIAL -20 1 x 0.3 ml Not Applicable
      NEBNext End Repair Enzyme Mix E6271AAVIAL -20 1 x 0.15 ml Not Applicable
      TE Buffer E6293AAVIAL -20 1 x 3.3 ml 0.1 X
      NEBNext DNA Library Primers for Ion Torrent™ E6291AAVIAL -20 1 x 0.5 ml Not Applicable
      T4 DNA Ligase Buffer for Ion Torrent™ E6276AAVIAL -20 1 x 0.5 ml Not Applicable
      Bst 2.0 WarmStart® DNA Polymerase E6277AAVIAL -20 1 x 0.05 ml Not Applicable
      NEBNext Q5 Hot Start HiFi PCR Master Mix E6629AAVIAL -20 2 x 1.25 ml 2 X

Properties & Usage

Materials Required but not Supplied

  • Agencourt® AMPure® XP Beads (Beckman Coulter, Inc., #A63881) or PCR Column Purification Kit (Qiagen or other)
  • 80% ethanol (freshly prepared)
  • Size selection materials [E-Gel® (Life Technologies, Inc.) or AMPURE XP Beads etc.]
  • Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)

Protocols, Manuals & Usage

Protocols

  1. NEBNext® Fast DNA Library Prep Set for Ion Torrent™

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

FAQs & Troubleshooting

FAQs

  1. What sequencing platform can I use the NEBNext Fast DNA  Library Prep Sets for Ion Torrent reagents for?
  2. What type of starting material can be used with NEBNext® DNA Library Prep Set for Ion Torrent?
  3. How much starting material do I need to use when preparing libraries using the NEBNext Fast DNA Library Prep Set for Ion Torrent?
  4. Does the NEBNext DNA Library Prep Sets for Ion Torrent work well for GC-rich DNA and AT-rich DNA?
  5. Can I use the NEBNext DNA Library Prep Sets for Ion Torrent reagents for preparing RNA?
  6. What methods for reaction clean up can be used with Ion Torrent Library Preparation Kits?
  7. What methods for size selection can be used with Ion Torrent Library Preparation Kits?
  8. Are these reagents available in a customized format, larger format or 96 well formats?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows. 

Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc.  New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the NEBNext® Fast DNA Library Prep Set for Ion Torrent™ for Research Use Only (RUO). Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com.

Trademarks

NEBNEXT®, NEW ENGLAND BIOLABS®, Q5® and WARMSTART® are registered trademarks of New England Biolabs, Inc.
LITMUS™ and THERMOPOL™ are trademarks of New England Biolabs, Inc.

AMPURE® is a registered trademark of Beckman Coulter, Inc.
BIOANALYZER® is registered trademark of Agilent Technologies, Inc.
E-GEL® is a registered trademark of Life Technologies, Inc.
ION XPRESS™, ION TORRENT™ and PGM™ are trademarks owned by Life Technologies, Inc.

Other Products You May Be Interested In