Product Class: Other

NEBNext® dA-Tailing Module

Product Introduction

This module is part of the original standard library prep workflow, which is longer than the NEBNext Ultra II DNA Library Prep Kit (NEB #E7645) workflow, has more components, and requires more clean-up steps. It also requires higher input amounts: 1 - 5 μg of input DNA vs. 500 pg - 1 μg required for the Ultra II DNA workflow.
Catalog # Size Concentration
E6053S 20.0 reactions
E6053L 100.0 reactions

Product Information

Description






 

The NEBNext dA-Tailing Module enables incorporation of a non-templated dAMP on the 3´ end of a blunt-ended DNA fragment. The module is optimized for use with the NEBNext Quick Ligation Module (NEB #E6056), and is part of the original standard DNA library prep workflow for Illumina sequencing, which is suitable for 1–5 µg of input DNA.

Each kit component must pass rigorous quality control standards, and for each new lot the entire set of reagents is functionally validated together by construction and sequencing of an indexed library on the Illumina sequencing platform.

This product can be used in the following applications:
A-tailing,
DNA Sequencing

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • E6053S     -20    
      NEBNext dA-Tailing Reaction Buffer E6055AVIAL -20 1 x 0.1 ml 10 X
      Klenow Fragment (3´→ 5´ exo–) E6054AVIAL -20 1 x 0.06 ml Not Applicable
  • E6053L     -20    
      NEBNext dA-Tailing Reaction Buffer E6055AAVIAL -20 1 x 0.5 ml 10 X
      Klenow Fragment (3´→ 5´ exo–) E6054AAVIAL -20 1 x 0.3 ml Not Applicable

Properties & Usage

Materials Required but not Supplied

 

Thermocycler

AMPure® XP Beads (Beckman Coulter, Inc. #A63881) or SPRIselect® Reagent Kit (Beckman Coulter, Inc. #B23317)

10 µM Tris-HCl, pH 7.5-8.0 or 0.1 µM Tris-HCl, pH 8.0

References

  1. Clark, J.M. et al. (1987). J. Mol. Biol.. 198(1), 123-127.
  2. Derbyshire, V. et al. (1988). Science. 240, 199-201.

Protocols, Manuals & Usage

Protocols

  1. NEBNext dA-Tailing Module Protocol (E6053)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

FAQs & Troubleshooting

FAQs

  1. What type of sample prep/library construction can I use the dA-Tailing Module reagents for?
  2. How much DNA can I dA-Tail in one NEBNext dA-Tailing Reaction?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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