Product Class: Kit

NEBNext® LV Unique Dual Index Primers Set 2


Catalog #E3402

Product Introduction

NEBNext LV Unique Dual Index Primers Set 2 includes 96 unique pairs of i5 and i7 index primers optimized for low-volume (LV) NEBNext workflows, including NEBNext EM-seq™ v2 and E5hmC-seq™, with Illumina® sequencing. The primers enable incorporation of unique index pairs during library amplification and have been designed to minimize index hopping.

  • Optimized for the low-volume (LV) NEBNext library prep workflows
  • Addresses the “barcode hopping”issue seen with some Illumina sequencing instruments
  • Increases sample identification specificity with unique dual index primer pairs

Product Information

Description

NEBNext LV Unique Dual Index Primers have been selected and paired for optimal multiplex index incorporation during PCR. Optimized for low-volume (LV) NEBNext workflows, including EM-seq v2 and E5hmC-seq, these primers enable multiplexing and have been designed to minimize index hopping. They are supplied as pairs of premixed i5 and i7 8-base index primers in a 96-well plate that has a pierceable foil seal for easy, single use.

The index sequences from NEBNext LV Unique Dual Index Primers Set 2 are compatible with and can be combined with the other NEBNext LV Unique Dual Index Primers sets to multiplex up to 480 reactions. Note: NEBNext LV Unique Dual Index Primers Set 2A (NEB #3390) and Set 2B (NEB # E3392) are 24-index subsets of Set 2; therefore, we do not recommend combining Set 2A and/or 2B with Set 2. Please refer to the relevant NEBNext library prep kit manual for usage instructions. Sample sheets for use with these NEBNext LV Unique Dual Index Primers can be found under "Usage Guidelines" on this product page (see below).

This product is related to the following categories:
NEBNext® Multiplex Oligos (Adaptors & Primers) Products,
Next Generation Sequencing Library Preparation Products,

Kit Components

Kit Components

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Protocols, Manuals & Usage

Protocols

  1. Where can I find protocols for use of the NEBNext® LV Unique Dual Index Primers Set 2, including index pooling guidelines? (NEB #E3402)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Usage & Guidelines

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. What is the difference between NEBNext® Primers for Epigenetics and NEBNext LV Unique Dual Index Primers?
  2. Are Sample Sheets available for use with the NEBNext® LV Unique Dual Index Primers Sets 1-5?
  3. Are libraries that have been dual indexed using the NEBNext® LV Unique Dual Index Primers compatible with single-end sequencing?
  4. How many primer pairs are available in the NEBNext® LV Unique Dual Index Primer Pairs Sets 1-5?
  5. Can the NEBNext® LV Unique Dual Index Primers be used to address “index hopping” with Illumina® patterned flow cells?
  6. Are the NEBNext® LV Unique Dual Index Primers the same as NEBNext Multiplex Oligos for Illumina®?
  7. Are the NEBNext® LV Unique Dual Index Primers validated for the EM-seq™ v2 or E5hmC-seq™ workflows?
  8. What is the concentration of the Index Primers in NEBNext® LV Unique Dual Index Primers?
  9. How many reactions’ worth of primer are in each well of the NEBNext® LV Unique Dual Index Primers?
  10. Can I use the 96-well primer plate supplied with the NEBNext LV Unique Dual Index Primers to do my PCR reactions?
  11. Do I need to spike in custom sequencing primers when sequencing libraries made with NEBNext® LV Unique Dual Index Primers on Illumina® sequencing instruments?
  12. Can I perform single read runs and still get both index sequences? Can I run both single read and paired-end recipes with dual-indexed libraries?
  13. What sequences need to be trimmed for NEBNext libraries that are sequenced on an Illumina instrument?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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