BL21 Competent E. coli
Product informationCode | Name | Size | Quantity | Price | |
---|---|---|---|---|---|
C2530H |
BL21 Competent E. coli |
20 x 0.05 ml | - | Unavailable in your region |
BL21 Competent E. coli
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Product Introduction
BL21 Competent E. coli is a widely used non-T7 expression E. coli strain and is suitable for transformation and protein expression. This strain does not express the T7 RNA Polymerase.
- Ideal for Plac, Ptac, Ptrc ParaBAD expression vectors
- Protease deficient
- No dry ice surcharge on competent cell shipments
- Outgrowth medium included
- Free of animal products
Catalog # | Size | Concentration |
---|---|---|
C2530H | 20.0 x 0.05 ml |
- Product Information
- Protocols, Manuals & Usage
- Tools & Resources
- FAQs & Troubleshooting
- Citations & Technical Literature
- Quality, Safety & Legal
- Other Products You May Be Interested In
Product Information
Description
Highlights
- Routine non-T7 expression
- Deficient in proteases Lon and OmpT
- Resistant to phage T1 (fhuA2)
- B Strain
- Free of animal products
Transformation Efficiency
1–5 x 107 cfu/μg pUC19 DNA
Genotype
fhuA2 [lon] ompT gal [dcm] ΔhsdS- This product is related to the following categories:
- Non-T7 Expression,
- E. coli Protein Expression Strains Products,
- Protein Expression Products,
- E. coli Expression Strains Products,
- This product can be used in the following applications:
- Non-T7 Expression,
- Protein Expression in E. Coli,
- Protein Expression
Reagents Supplied
Reagents Supplied
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration | |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Properties & Usage
Antibiotic for Plasmid Selection
Antibiotics for Plasmid Selection | Working Concentration |
---|---|
Ampicillin | 100 µg/ml |
Carbenicillin | 100 µg/ml |
Chloramphenicol | 33 µg/ml |
Kanamycin | 30 µg/ml |
Streptomycin | 25 µg/ml |
Tetracycline | 15 µg/ml |
Shipping Notes
- Ships on dry ice
Features
- Routine non-T7 expression
Related Products
Companion Products
Materials Sold Separately
Product Notes
- CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
- STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.
- COMPATIBLE EXPRESSION VECTORS/PROMOTERS: Ptac, Ptrc, Plac, PlacUV5, ParaBAD, PrhaBAD, T5-lacO
Note: Expression from vectors containing a T5-lacO promoter will not be regulated unless the expression vector encodes the lacI gene or a compatible vector expresses LacI or the host strain overproduces LacI (e.g NEB #C3037 NEB Express Iq Competent E. coli)."
Protocols, Manuals & Usage
Protocols
Usage & Guidelines
Tools & Resources
Selection Charts
FAQs & Troubleshooting
FAQs
- Does New England Biolabs offer strains of Competent E.coli suited for protein expression?
- What are the solutions/recipes (C2530)?
- What are the strain properties (C2530)?
- Why are there no colonies or no growth in liquid culture (C2530)?
- Why is the induced protein insoluble (C2530)?
- Why is there no protein visible on gel or no activity (C2530)?
- Can I store competent cells at -20°C instead of -80°C?
- Which kind of transformation tubes should be used?
- What volume of DNA can be added into competent cells?
- What is the shelf life for this strain (NEB #C2350H)?
- Are NEB's competent cells compatible with the “Mix & Go" protocol?
- What is the difference between BL21 and BL21(DE3) competent E.coli cells?
- How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase it as a stand alone product, it recommends storing it at 4°C. Which is better?
Citations & Technical Literature
Citations
Additional Citations
Quality, Safety & Legal
Quality Assurance Statement
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]Certificate Of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]- C2530H_v1_0221608
- C2530H_v1_0231609
- C2530H_v1_0251610
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- C2530H_v1_10252585
- C2530H_v1_10257591
Safety DataSheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.BL21 Competent E. coli
pUC19 Vector
SOC Outgrowth Medium
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Other Products You May Be Interested In
The supporting documents available for this product can be downloaded below.
Protocol
Transformation Protocol (C2530)
Protocol
Transformation Protocol (NEB #C2530)
Protocol
Transformation Protocol (C2530)
Protocol
Transformation Protocol (NEB #C2530)