Product Class: Kit

Blue Protein Loading Dye

Product Introduction

The blue protein loading dye contains one vial of blue loading buffer and one vial of 30X reducing agent. The combined solution is ideal for protein gel applications.

  • 1X Blue Loading Buffer Composition: 62.5 mM Tris-HCl (pH 6.8), 2% (w/v) SDS, 10% glycerol, 0.01% (w/v) bromophenol blue
  • 30X Reducing Agent: 1.25 M DTT
  • SDS interacts with positively charged amino acids of proteins, thereby disrupting interactions that form protein structures to separate proteins based on size rather than charge or shape 
  • DTT is present to help reduce structural disulfide bonds 
Catalog # Size Concentration
B7703S 8.0 ml

Product Information

Description

Blue Loading Buffer Reagents for SDS-PAGE:

3X SDS Blue Loading Buffer (8 ml):
187.5 mM Tris-HCl, pH 7.0
6% SDS (w/v)
30% glycerol
0.03% bromophenol blue (w/v)

30X Reducing Agent (1 ml):
1.25 M DTT
(Store at -20°C)
This product is related to the following categories:
Protein Tools Buffers and Diluents Products,
Protein Standards Products,
Gel Loading Buffers,
Protein Standards Products,
Buffers Products,

Kit Components

Pack Components

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • B7703S     -20    
      Blue Protein Loading Dye B7703SVIAL -20 1 x 8 ml 3 X
      30X Reducing Agent B7705SVIAL -20 1 x 1 ml 30 X

Properties & Usage

Product Notes

  1. Reagents stored at different temperatures.
  2. Prepare fresh 3X Reducing Blue Loading Buffer by adding 1/10 volume 30X Reducing Agent to 1 volume of 3X Blue Loading Buffer.
  3. Prepare samples by adding 2 volumes of Sample to 1 volume 3X Reducing Blue Loading Buffer (from step 1).
  4. Heat samples to 95-100°C for 3-5 minutes.
  5. After a quick microcentrifuge spin, load directly on to a gel. To ensure uniform mobiity, load an equal volume of 1X Reducing Blue Loading Buffer into any unused wells.

Protocols, Manuals & Usage

Protocols

  1. Use of the 3X Reducing Blue Protein Loading Dye for Protein Gel Samples (NEB #B7703)

FAQs & Troubleshooting

FAQs

  1. What is supplied with the Blue Loading Buffer pack?
  2. What is the composition of the Blue Loading Buffer?
  3. How should the Blue Loading Buffer be used?
  4. What is the difference between the Red and Blue Loading Buffers?
  5. What are the functions of the buffer pack components?

Tech Tips

DTT will degrade at room temperature. Keep the two components separate at store at their proper temps.

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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