NucleoMag 96 Blood
Product informationBenefits
- One-tube procedure minimizes risk of cross-contamination
- Small elution volumes: ≥ 50 µL (NucleoMag® Blood 200 µL), ≥ 1 mL (NucleoMag® Blood 3 mL)
- Complete processing possible at room temperature
- Easily adapted to automated use
- Consistently high yields
| Code | Name | Size | Quantity | Price | |
|---|---|---|---|---|---|
744501.1 |
NucleoMag 96 Blood 200 ul |
1 x 96 preps | - | Unavailable in your region | |
744501.4 |
NucleoMag 96 Blood 200 ul |
4 x 96 preps | - | Unavailable in your region | |
744502.1 |
NucleoMag 96 Blood 3ml |
1 x 96 preps | - | Unavailable in your region |
Principle
Magnetic-bead based isolation of genomic DNA from whole blood
NucleoMag® Blood 200 µL / 3 mL is designed for the rapid parallel purification of genomic DNA from whole blood in a 96 or 24-well format. After lysis of the blood cells genomic DNA is selectively bound to the NucleoMag® B-Beads. Contaminants are washed away and the DNA can be eluted from the beads under low-salt conditions. As the magnetic bead technology provides a closed system (no transfer of sample from one well to another is necessary during the preparation) the risk for cross-contamination is reduced.
The NucleoMag® technology can be easily adapted to common liquid handling instruments. It can be used either with static-pin separators (e.g., NucleoMag® SEP) or magnetic separators integrated into robotic workstations.
Features
| NucleoMag® Blood 200 µl | NucleoMag® Blood 3 ml | |
| Technology | Magnetic-bead technology | |
| Format | Highly reactive superparamagnetic beads | |
| Processing | Manual or automated | Automated* |
| Sample material | 200 µl whole blood (fresh or frozen, EDTA or citrate treated) |
3 ml whole blood (fresh or frozen, EDTA or citrate treated) |
| Fragment size | 20–approx. 50 kbp | 20–approx. 50 kbp |
| Typical yield | 2–8 µg | 100–130 µg |
| A260/A280 | 1.6–1.9 | 1.6–1.9 |
| Elution volume | ≥ 50 µl | ≥ 1 ml |
| Preparation time | 45 min/ 96 preps* | 60 min/24 preps* |
| Binding capacity | Approx. 0.4 µg/µl | Approx. 0.4 µg/µl |
* Established on KingFisher® Flex
For more detailed information and documents, click here.
The supporting documents available for this product can be downloaded below.