New generation for efficient and quick RNA isolation from cells and tissues

• NucleoSpin^® gDNA Removal Column – no time consuming rDNase digestion
• Convenient handling – lysate clearing and gDNA removal with one column in one step
• New efficient lysis buffer – no b-mercapthoethanol or TCEP necessary

The NucleoSpin^® RNA Plus kit integrates a new feature, the NucleoSpin® gDNA Removal Column. This Mini spin column removes quickly and efficiently genomic DNA without the need of DNase digestion.

In the first step cells and tissues are lysed without the need of b-mercapthoethanol or TCEP. The chaotropic salt included in the lysis buffer immediately inactivates RNases. The lysate is added to the NucleoSpin® gDNA Removal Column to clarify the lysate and to remove contaminating gDNA. After addition of the binding solution to the flow-through, the RNA is bound to the NucleoSpin® RNA Plus Column. Afterwards, two washing steps remove salts, metabolites, and macromolecular cellular components. High quality RNA is eluted with RNase-free H₂O.
 

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Applications

• RNA isolation from cultured cells, animal tissue, plant tissue, microorganisms
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays
   

Read this publication in Nature Communications about the successful RNA purification for downstream RNA sequencing with the NucleoSpin RNA Plus Kit from MACHEREY-NAGEL
 

NucleoSpin RNA Plus procedure

Application data

Efficient RNA recovery
RNA was isolated from HeLa cells (5 x 10⁶) and mouse kidney (20 mg) with the NucleoSpin® RNA Plus (blue) and a competitor kit (red). The RNA recovery was quantified by qRT-PCR. Lower CT values indicates higher RNA yields.
 

Reliable genomic DNA removal
NucleoSpin® RNA Plus (blue) and a competitor kit (red) have been used for the isolation of RNA from HeLa cells (5 x 10⁶) and mouse kidney (20 mg). Residual genomic DNA were measured by qPCR. The MN kit shows higher CT values indicating a more efficient genomic DNA removal. Higher CT values mean less genomic DNA.


 

RNA of high integrity
10 mg mouse kidney tissue was used for RNA isolation. Equal amount of RNA were analyzed on an Agilent Bioanalyzer. The RIN value is higher for the MN kit.

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