Principle

Isolation of genomic DNA from food and feed in flexible 8-well strip format and for high throughput in approved 96-well format

Identification of GMO-DNA or animal components in food and feed has become an important application of public interest. Food regulation in many markets requires mandatory labeling for GMO containing food. They can be distinguished from conventional products by analytical methods, for example real-time PCR. Food samples are very heterogeneous and contain many different compounds like fat, cocoa, or polysaccharides which can lead to suboptimal extraction or faillure during subsequent processing of DNA. The NucleoSpin® Food system guarantees good recovery rates even for small genomic DNA fragments (< 1 kb) out of processed, complex food matrices, which generally have very low DNA contents as well as poor quality, degraded DNA. After the food samples have been homogenized, the DNA can be extracted with lysis buffers containing chaotropic salts, denaturing agents and detergents. The standard isolation ensures lysis using buffer CF, which was especially developed in cooperation with GEN-IAL, Troisdorf, Germany (patent pending). Lysis mixtures should be cleared by centrifugation or filtration in order to remove contaminants and residual cellular debris. The clear supernatant is then mixed with binding buffer and ethanol to create conditions for optimal binding to the NucleoSpin® silica membrane, which was selected for this purpose due to its unique DNA-binding properties. Resulting eluates are readyto-use in all types of subsequent detection methods, especially in real-time and basic PCR technologies and can be used for the identification of GMO-DNA or animal components in food and feed. The NucleoSpin® 8 / 96 Food system allows medium to high-throughput purification of DNA from food samples. The optimized 8-well strip respectively 96-well block design allows a high performance and easy handling of multiple samples. NucleoSpin® 8 / 96 Food can be processed by centrifugation or under vacuum. Processing under vacuum allows easy automation on common liquid handling instruments.

Features