NucleoSpin RNA Midi
Product informationBenefits
- Efficient removal of genomic DNA – rDNase included for on-column digestion*
- Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters L (shredders) included
- Up to 600 μg ready-to-use RNA
* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA Midi system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:
– the number of DNA copies per preparation: single copy target < plastidial/ mitochondrial target < cells transfected with plasmid
– decreasing PCR amplicon size
| Code | Name | Size | Quantity | Price | |
|---|---|---|---|---|---|
740962.20 |
NucleoSpin RNA Midi (20) |
20 preps | - | Unavailable in your region |
NucleoSpin® RNA Midi allows isolation of DNA-free high-quality RNA from large amounts of starting material, e.g. cultured cells, tissue, bacteria, and yeast. The working procedure is similar to the NucleoSpin® RNA II method and includes effective rDNase digestion of contaminating DNA during the purification process.