Principle

All-round kit with highest sensitivity for purification of total DNA from clinical or forensic samples, tissues, cells, yeast, bacteria, or viruses

NucleoSpin® Tissue is designed for the rapid purification of highly pure genomic DNA from tissue samples, mouse tails, bacteria, yeast, forensic samples (hair, dried blood spots, buccal swabs, cigarette filters), and clinical samples (stool, urine). Up to 35 μg of high-purity genomic DNA can be prepared (typical yields from tissue or cells: 15–25 μg). The obtained DNA can be used directly for PCR, Southern blotting, or any kind of enzymatic reaction. With the NucleoSpin® Tissue method, lysis is achieved by incubation of the samples in a solution containing SDS and Proteinase K at 56 °C. Appropriate conditions for binding of DNA to the silica membrane of the NucleoSpin® Tissue Columns are created by addition of large amounts of chaotropic ions (Binding Buffer B3) and ethanol to the lysate. The binding process is reversible and specific to nucleic acids. Contaminations are removed by efficient washing with buffer. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer and is ready to use for subsequent reactions.

Features

Applications*

• DNA from tissue (e.g., mouse tails)
• DNA from cells (e.g., cultured human or animal cells, bacteria, yeast)
• DNA from clinical samples (e.g., stool, urine, biopsy samples)
• DNA from forensic samples (e.g., dried blood spots, hair, buccal swabs, cigarette stubs)
• DNA from FFPE tissue
• DNA from blood sample storage cards (e.g., NucleoCard®)
• Typical downstream applications: multiplex PCR, Southern blotting, enzymatic reactions

* Kits to be used for research purposes only

Protocols are available for the isolation of

Genomic DNA, e.g. from

• human or animal tissue
• mouse or rat tails
• bacteria
• yeast
• dried blood spots (Guthrie cards) -
• hair roots
• paraffin-embedded tissue
• stool
• insects
• dental swabs
• buccal swabs

Viral DNA, e.g.

• CMV from urine
• CMV from stool

Bacterial DNA, e.g.

• Mycobacterium tuberculosis or Legionella pneumophila from sputum or bronchoalveolar lavage
• EHEC bacteria from food (e.g., fresh cows' milk)
• Chlamydia trachomatis from cultures, biological fluids, or clinical specimens
• Borrelia burgdorferi from urine