Principle

For rapid preparation of viral DNA from blood and biological fluids

The isolation of viral DNA – especially from Hepatitis B (HBV) – from serum and plasma has become more important with the growing demands of clinical diagnostics. The extraction of HBV DNA is demanding due to the special genome organization of the Hepatitis B virus. A terminal protein, covalently bound to one of the HBV DNA strands, has to be removed prior to binding of the nucleic acid to the silica membrane. The NucleoSpin® Blood method includes a Proteinase K step for digestion of proteins. Subsequently, the viral DNA is bound to the silica membrane and washed twice with two different buffers ensuring removal of contaminations. Elution can be performed with water or low-salt buffer. The following figures show the outstanding sensitivity of the HBV DNA purification with NucleoSpin® Blood.

Features