NucleoSpin Plant II
Product informationBenefits
- Two alternative lysis buffers included for optimal processing of various samples and highest flexibility:
Lysis Buffer PL1, based on CTAB lysis method
Lysis Buffer PL2, based on SDS lysis method - NucleoSpin® Filters included for clarification of lysate
- Improved buffer compositions → higher yield and purity
- Optimized silica membrane → improved DNA binding
- RNase A included
| Code | Name | Size | Quantity | Price | |
|---|---|---|---|---|---|
740770.10 |
NucleoSpin Plant II |
10 preps | - | Unavailable in your region | |
740770.50 |
NucleoSpin Plant II |
50 preps | - | Unavailable in your region | |
740770.250S |
NucleoSpin Plant II Columns |
250 Columns | - | Unavailable in your region | |
740770.250 |
NucleoSpin Plant II |
250 preps | - | Unavailable in your region |
Principle
The second generation for rapid isolation of genomic DNA from plant samples with higher yield and quality
With NucleoSpin® Plant II MACHEREY-NAGEL introduces the second generation of kits for isolation of gDNA from plant material, fungi, and other biological samples. The kit includes two optimized, alternative lysis buffers based on the established CTAB and SDS lysis methods. As plants are very heterogenous and contain a lot of different metabolites like polyphenols, polysaccharides, or acidic components, NucleoSpin® Plant II offers two different lysis procedures for optimal processing of various samples. The silica membrane in the NucleoSpin® Plant II Columns is optimized to improve DNA binding. NucleoSpin® Filters are included for convenient clarification of lysates. NucleoSpin® Plant II allows processing of up to 100 mg (wet weight) or 20 mg (dry weight) starting material. Depending on the individual sample typical yields are in the range from 1 to 30 μg DNA. The eluted DNA is ready to use for subsequent reactions like PCR, restriction analysis, and others.
Features
| Technology | Silica-membrane technology |
| Format | Mini spin columns |
| Sample material |
<100 mg plant tissue (wet weight), <20 mg plant tissue (dry weight) |
| Lysate clarification | NucleoSpin® Filters |
| Fragment size | 50 bp - approx. 50 kbp |
| Typical yield | 1-30 µg |
| A260/A280 | 1.6-1.9 |
| Elution volume | 2 x 50 µl |
| Preparation time | 30 min/prep |
| Binding capacity | 50 µg |
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The supporting documents available for this product can be downloaded below.