Principle

Isolation of viral RNA and DNA in flexible 8-well strip format and high throughput in 96-well format

NucleoSpin® 8 / 96 Virus is designed for the rapid simultaneous preparation of viral RNA and DNA from serum, plasma, or any cell-free biological fluid. The kits allow reliable purification of viral RNA and DNA without cross-contamination. NucleoSpin® 8 Virus and NucleoSpin® 96 Virus are primarily designed for manual use in a centrifuge. NucleoSpin® 8 / 96 Virus Core Kits allow for more variation and higher flexibility in the consumables used for lysis, washing, and elution and are primarily recommended for manual or automated vacuum use. With the NucleoSpin® 8 / 96 Virus method, RNA viruses are lysed quickly and efficiently by a lysis buffer which is a highly concentrated solution of GITC. DNA viruses (e.g., HBV) are usually more difficult to isolate and require a digestion of samples with Proteinase K. Lysis buffer and ethanol create appropriate conditions for binding of nucleic acids to the silica membrane of the NucleoSpin® Virus Binding Strips or Plate. Carrier RNA included in lysis buffer improves binding and recovery of the low concentrated viral RNA / DNA. Contaminations (potential PCR inhibitors) like salts, metabolites, and soluble macromolecular cellular components are removed in washing steps with ethanolic wash buffers. The purified viral nucleic acids can be eluted in low salt buffer or water and are ready-to-use in subsequent downstream applications like RT-PCR or PCR.

Features