PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume

The NucleoSpin® Gel and PCR Clean-up procedure is the easiest way to purify DNA fragments from agarose gels as well as for direct purification of PCR products. The kit includes one buffer for both applications, which contains a pH indicator displaying you the correct pH for optimal kit performance. The purification procedure from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities. The NucleoSpin® Gel and PCR Clean-up Columns provide convenient performance for PCR clean-up: After addition of Binding Buffer NTI the mixture is applied onto the silica membrane. Contaminations are removed by a simple washing step with ethanolic Buffer NT3. For gel extraction the agarose gel slice is dissolved in high-salt Buffer NT and applied to a NucleoSpin® Gel and PCR Clean-up Column followed by centrifugation and a subsequent washing step. Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline Buffer NE (5 mM Tris/HCl, pH 8.5).

Technology	Silica-membrane technology
Format	Mini spin columns
Sample material	<200 µl PCR reaction mixture <200 mg TAE/TBE agarose gel
Fragment size	50 bp-20 kbp
Typical recovery	70-95%
A260/280	1.8-1.9
Elution volume	15-50 µl
Preparation time	10 min/6 preps
Binding capacity	25 µg

 

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