Principles

The anion exchanger for purification of P1, BACs, PACs, and other large constructs!

Due to the high selectivity of NucleoBond® AX, this anion exchanger is ideally suited for the purification of very large constructs like P1, BACs and PACs. Using our NucleoBond® BAC 100 kit, it takes less than 1 hour to isolate e.g. BAC DNA free from any kind of RNA, proteins, metabolites, dyes or carbohydrates. After purification, all nucleic acids show absorption ratios of A260/A280 as high as 1.8–2.0. Low amounts of impurities, which often act as strong inhibitors for enzymatic processing, transfections, transcriptions, etc., are removed completely. The high purity of plasmid DNA after purification using NucleoBond® AX is illustrated by the agarose gels shown.

Features