Combine NucleoZOL with proven NucleoSpin® technology
 

• Total RNA (incl. miRNA) with a simple bind-wash-elute procedure
• Superior RNA yields due to the efficient NucleoZOL* lysis
• Save time and benefit from the easy and proven handling

*NucleoZOL not included

 
Applications**

• RNA purification with NucleoZOL* lysis and well-known NucleoSpin® RNA Columns

** Kits to be used for research purposes only
 

Principle/Procedure

NucleoSpin® RNA Set for NucleoZOL is designed for combining the one-phase RNA isolation reagent NucleoZOL with silica spin columns. After lysing the sample in NucleoZOL (not provided in the mini spin kit) and pelleting contaminating DNA and proteins, the supernatant is mixed with an optimized binding buffer for efficient binding of small and large RNA to the silica membrane. A convenient bind-wash-elute procedure allows highly efficient isolation of pure RNA (small and large RNA).

NucleoSpin® RNA Set for NucleoZOL procedure (after NucleoZOL lysis)

Application data

    
Total RNA was isolated with NucleoZOL combined with mini spin columns (NucleoSpin® RNA Set for NucleoZOL) or with a competitor product, that combines a liquid extraction with a column based method (competitor Z). Total RNA yields were quantified using an Agilent 2100 Bioanalyzer™.
 

Total RNA was isolated with NucleoZOL in combination with the NucleoSpin® RNA Set for NucleoZOL from 5 x 106 HeLa cells. RNA was analyzed on an Agilent Bioanalyzer™, a RIN of 9.8 indicates perfect RNA quality.