HighPrep DTR

Magnetic beads based reagent for manual and automated Sanger sequencing reaction clean-up for both ABI and MegaBACE sequencing platforms.

APPLICATIONS

• Sanger sequencing reaction clean-up / dye terminator removal for:
• Sanger sequencing on ABI platforms
• Sanger sequencing on MegaBACE platforms

BENEFITS

• Rapid and reliable clean-up of sequencing reactions
• High DNA recovery yields, long sequence readings
• Flexible - can be used in any format
• No centrifugation step, no filtration step
• Adaptable to common liquid handling workstations

The HighPrep DTR is a high performance paramagnetic bead-based reagent for BigDye™ terminator removal from Sanger sequencing reactions for reliable and long readings of DNA sequences. The HighPrep DTR consists of a selective binding of DNA to the paramagnetic beads, followed with washing off nucleotides, primers, and non-targeted amplicons, and finally elution of pure DNA. HighPrep DTR is an excellent choice for both manual and fully automated purification of sequencing reaction products. The protocol can be adapted to common liquid handling workstation.

BigDye is a registered trademark of Applied Biosystems

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