HighPrep Viral DNA & RNA Kit

Magnetic beads based kit for rapid isolation of total viral nucleic acids from whole blood, serum, plasma, saliva and other body fluids. OPTIMIZED FOR ISOLATION OF SARS-CoV-2 RNA

APPLICATIONS

• Viral RNA and DNA isolation for:
• RT-qPCR; RT-PCR, PCR
• One-Step RT-qPCR
• Virus detection, genotyping
• Viral load monitoring,

BENEFITS

• OPTIMIZED FOR ISOLATION OF SARS-CoV-2 RNA
• Rapid and reliable purification of viral nucleic acids
• Adaptable to various automated liquid handling workstations
• No toxic organic solvents

The HighPrep Viral DNA & RNA kit is designed for rapid and reliable isolation of total viral nucleic acids from whole blood, serum, plasma, saliva and other body fluids as well as nasopharyngeal swabs soaked in virus transport media or other buffers. This kit is highly efficient in vIral nucleic acid isolation and the extracted RNA (and DNA) is suitable for direct use in most downstream applications such as one-step RT-qPCR, RT-PCR, PCR, nucleic acid amplification, cloning, sequencing, and enzymatic reactions. The kit can be used in low throughput manual workflows and is also adaptable to majority of the liquid handling workstations in the market.

Protocols

• This is a short overview of the protocol, for all detailed protocols, please refer to PRODUCT DOCUMENTS
• Add the sample fluid into the well or tube.
• Add Viral Lysis Buffer, Proteinase K and incubate at 56-60°C to perform cell lysis.
• Add NBE, isopropanol (Not Supplied) and MAG-S1 particles. Incubate and bind to nucleic acids
• Perform one wash step with RDW Buffer (Mixed with 100% ethanol)
• Wash the beads 2 times with 70% ethanol (Not Supplied)
• Perform nucleic acid elution using supplied Nuclease-Free Water
• Store NA at -20°C

Features	Specifications
Main functions	Isolation of Viral RNA from various sample matrices
Starting material (Sample matrix)	Whole blood, saliva, swabs, plasma, samples collected in Viral Transport Media, serum
Sample amount (Input)	can be scaled up
Downstream application	Real time quantitative PCR, Reverse Transcriptase PCR, PCR, NGS
Process method	Manual or automation
Instrument	Adaptable to most nucleic acid purification instruments but a ready-made script for Kingfisher Flex is available upon request
Purification method	Magnetic bead-based technology
Purification time	23 min on Kingfisher Flex Instrument

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