Amersham Typhoon NIR

Amersham Typhoon NIR laser scanner precisely quantitates up to two proteins, nucleic acids, or other biomolecules detected with NIR labeled antibodies.

Advantages

• Versatility: image multifluorescent-labeled on gels, membranes, microplates, culture dishes, and tissue sections*
• Infrared fluorescence imaging: use near-infrared (NIR) excitation to image IRDye™ and other infrared dyes
• Accurate quantitation: detect signals from very low levels (pg) of protein and differences across a dynamic range with greater than 5 orders of magnitude
• High resolution: resolve fine details in your sample with a pixel resolution of 10 µm
• High sample throughput: maximize efficiency using 40 × 46 cm scanning area to simultaneously image up to 20 gels or blots (10 × 8 cm) or 9 multiwell plates
• Ease of use: auto- and semi auto-scan functions, automatic filter recognition, and modular access make system well suited to multiuser environments
• Open data format: output image files are compatible with other open platform analysis software, such as ImageJ

* Depending on the sample format, additional accessories might be needed for the best performance.

Infrared fluorescent gel imaging system

Amersham Typhoon NIR is a laser scanner for precise quantitation of biomolecules in gels, blots, and other sample types. It has the same capabilities as the Typhoon NIR Plus model, except it does not have green fluorescent imaging functionality. Typhoon NIR supports the following imaging modes:

• Near-infrared fluorescent imaging for NIR fluorescent Western blotting and other applications
• Imaging of multiplex fluorescent Western blots, using fluorophore-labeled antibodies

Near-infrared fluorescent imaging

This infrared fluorescent gel imaging system is equipped with 2 NIR lasers that enable NIR fluorescent Western blotting. The two NIR lasers, optimized optics, and a new detector enable multiplex Western blotting with high sensitivity and wide dynamic range.

Multiplex fluorescence imaging applications

This Typhoon imager is optimized for multifluorescent imaging and precise quantitative Western blotting. Typhoon NIR supports multiplex fluorescence imaging with NIR channels.

Upgrade paths

This Typhoon imager is fully upgradable to the Amersham Typhoon NIR Plus or 5 models. Alternatively, red, blue, green, and phosphor imaging functionality can be added separately or in combination.

Specifications

Parameter	Amersham Typhoon NIR
Bit Depth	16-bit
Depth	800 mm
Detection Modes	Fluorescence
Dynamic Range	> 5 orders of magnitude
Frequency	50/60 Hz
Height	400 mm
Lasers	LD685, LD785
Operating Humidity	20% to 70%, RH (no dew condensation)
Pixel Size	10, 25, 50, 100 or 200 μm and a 1000 μm pre-scan
Power consumption	Approx. 0.3 kVA
Scanning Area	40 x 46 cm
Standard Filters	IRshort Fltr 720BP20, IRlong Filtr 825BP30
Voltage	AC100—240V~, Voltage variation±10%
Weight	93 kg
Width	900 mm
Model	Typhoon NIR

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