Principle

Magnetic-bead based PCR clean-up


For the parallel purification of PCR products NucleoMag® 96 PCR provides a reliable, cost- and labor-efficient tool. PCR products are selectively bound to the NucleoMag® PCR beads while contaminants (e.g., primers, dNTPs, enzymes, salts) are washed away. Purified PCR products are eluted from the beads under low-salt conditions and are ready-to-use in downstream applications like sequencing or microarray spotting. As the magnetic bead technology provides a closed system (no transfer of sample from one well to another is necessary during the preparation) there is no risk of cross-contamination. The NucleoMag® technology can be easily adapted to common laboratory automation platforms. It can be used either with static-pin separators (e.g., NucleoMag® SEP) or magnetic separators integrated into robotic workstations.

NucleoMag principle

DNA Contaminants NucleoMag Beads NucleoMag Beads are added to the sample, e.g. lysed blood cells, PCR reactions

DNA is bound to the NucleoMag Beads. Beads are held in the well by the magnet while contaminants are washed away

DNA is eluted from the beads and recovered, while beads are held in the well by the magnet. DNA is ready-to-use in downstream applications

Features

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