NucleoSpin RNA
Product informationBenefits
- Efficient removal of contaminating DNA – rDNase included for on-column DNA digestion*
- Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters (shredders) ncluded
- Up to 70 μg ready-to-use RNA
- Parallel purification of genomic DNA possible by using the NucleoSpin® RNA/DNA Buffer Set
* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:
– the number of DNA copies per preparation: single copy target < plastidial / mitochondrial target < cells transfected with plasmid
– decreasing PCR amplicon size
| Code | Name | Size | Quantity | Price | |
|---|---|---|---|---|---|
740955.10 |
NucleoSpin RNA |
10 preps | - | Unavailable in your region | |
740955.50S |
NucleoSpin RNA II Columns |
50 Columns | - | Unavailable in your region | |
740955.50 |
NucleoSpin RNA |
50 preps | - | Unavailable in your region | |
740955.250S |
NucleoSpin RNA Columns |
250 Columns | - | Unavailable in your region | |
740955.240C |
NucleoSpin RNA for QIAcube |
240 preps | - | Unavailable in your region | |
740955.250 |
NucleoSpin RNA |
250 preps | - | Unavailable in your region |
Formerly known as 'NucleoSpin RNA II'.
Re-branding NucleoSpin RNA II: not only a renaming, it's improved!
Principle
Mini spin kit for the isolation of RNA of highest integrity
NucleoSpin® RNA is recommended for isolation of total RNA from cultured cells, tissue, bacteria, yeast, cell-free biological fluids, and reaction mixtures. This kit allows purification of up to 70 μg of highly pure, DNA-free total RNA. As reference RNA from 106 cultured HeLa cells is prepared following the standard protocol resulting in 15 to 20 μg, but at least 10 μg of total RNA. It is possible to detect transcripts from very low amounts of cells. Total RNA prepared with NucleoSpin® RNA is suitable for applications like reverse transcriptase-PCR (qRT-PCR), Northern blotting, primer extension, array technology or RNase protection assays. For isolation of high-quality RNA it is important to prevent degradation of the RNA and to eliminate genomic DNA. With the NucleoSpin® RNA method, cells are lysed by incubation in a solution containing large amounts of chaotropic ions (see procedure). This lysis buffer immediately inactivates RNases – which are present in virtually all biological materials – and creates appropriate binding conditions which favour adsorption of RNA to the silica membrane. After lysis, homogenization and reduction of viscosity are achieved by filtration with NucleoSpin® Filter units provided with the kit.
Features
| Technology | Silica-membrane technology |
| Format | Mini spin columns |
| Sample material | < 5 x 106 cultured cells < 109 bacterial cells, up to 108 yeast cells < 30 mg tissue |
| Fragment size | > 200 b |
| Typical yield | 14 µg from 106 HeLa cells 70 µg from 109 bacterial cells |
| A260/280 | 1.9-2.1 |
| Typical RIN (RNA integrity number) | > 9 |
| Elution volume | 40-120 µl |
| Preparation time | 30 min/6 preps |
| Binding capacity | 200 µg |
For more detailed information and documents, click here.
The supporting documents available for this product can be downloaded below.