NucleoSpin 8 / 96 Soil
Product informationBenefits
- High quality DNA from diverse soil samples
- Different lysis buffer options for optimal processing of various soil sample composition
- Ceramic beads in bead tubes for most efficient lysis and no cross-contamination during lysis
- NucleoSpin® Inhibitor Removal Strips/Plate for eliminating all PCR inhibitors, such as humic substances
| Code | Name | Size | Quantity | Price | ||||
|---|---|---|---|---|---|---|---|---|
740779 |
NucleoSpin 8 Soil (12x8) |
12 x 8 preps |
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€ 446.00 |
+
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740787.2 |
NucleoSpin 96 Soil |
2x96 |
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€ 819.00 |
+
|
|||
740787.4 |
NucleoSpin 96 Soil |
4x96 |
|
€ 1434.00 |
+
|
Principle
Isolation of genomic DNA from soil and sediments
The NucleoSpin® 8 / 96 Soil kits allow manual and automated purification of DNA from soil samples using proven silica-membrane technology. The kit includes two alternative lysis buffers and an additional Enhancer SX. The option of two buffers and an enhancer allows tuning the optimal lysis conditions for highest yield from different soil samples of different compositions. Soil samples exhibit different pHs, contain different amounts of organic or anorganic matter, such as humic substances or polysaccharides. The lysis options and the unique NucleoSpin® Inhibitor Removal Strip/Plate provide a removal of all PCR inhibitors during the purification procedure. The NucleoSpin® Bead Tubes, containing ceramic beads, allow a most efficient lysis of microorganisms in the sample. The maximum amount of 500 mg soil as starting material yields typically 2- 10 µg DNA. The eluted genomic DNA is ready to use for downstream applications, such as PCR, qPCR, restrictions analysis, and others. Diluting the eluated DNA is not necessary. The NucleoSpin® 8 / 96 Soil kits are for manual and automated use.
Features
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NucleoSpin® 8 Soil |
NucleoSpin® 96 Soil |
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Technology |
Silica-membrane technology |
Silica-membrane technology |
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Format |
8-well strip |
96-well plates |
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Processing |
Manual or automated, vacuum or centrifugation |
Manual or automated, vacuum or centrifugation |
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Sample material |
< 500 mg soil, sludge, or sediment |
< 500 mg soil, sludge, or sediment |
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Fragment size |
50 bp–approx. 50 kbp |
50 bp–approx. 50 kbp |
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Typical yield |
2–10 µg (500 mg soil) |
2–10 µg (500 mg soil) |
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A260/A280 |
1.6–1.8 |
1.6–1.8 |
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Elution volume |
100–200 µL |
100–200 µL |
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Preparation time |
150 min/6 strips |
150 min/plate |
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Binding capacity |
50 µg |
50 µg |
For more detailed information and documents, click here.
The supporting documents available for this product can be downloaded below.