The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
N3019S     -20       λ DNA-Mono Cut Mix N3019SVIAL -20 1 x 0.1 ml 500 µg/ml   Gel Loading Dye, Purple (6X), no SDS B7025SVIAL 25 1 x 1 ml 6 X

Companion Products

• λ DNA-HindIII Digest
• λ DNA-BstEII Digest
• Gel Loading Dye, Purple (6X), no SDS

Materials Sold Separately

• Gel Loading Dye, Purple (6X), no SDS

1. These fragments are best separated by Pulsed Field Gel Electrophoresis. Alternatively Lambda Mono Cut Mix can be separated by conventional gel electrophoresis using the following conditions: 0.5 μg Lambda Mono Cut Mix, 0.4 % agarose gel, 1X TAE, 20-30 V, 25°C for 24 hours.
2. 1X Gel Loading Dye, Purple, no SDS: 
   2.5% Ficoll®-400
   10mM EDTA
   3.3mM Tris-HCl (pH 8.0@25°C)
   0.02% Dye 1
   0.001% Dye 2

1. Daniels, D.L. et al. (1983). Appendix II:Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.

1. Suggested Loading Protocol for DNA Ladders & Markers

    

2. Suggested Loading Protocol for DNA Ladders & Markers

1. How can I quantify the amount of DNA in each band of a marker?
2. General information on Lambda DNA Mono Cut Mix:
3. Can I use Midori Green with the DNA Ladders from NEB?
4. Can I use SYBR^® and/or GelRed^® dyes with the DNA Ladders from NEB?
5. Why is my DNA Ladder floating out the wells and Why are there no bands visible in my DNA ladder gel lane?
6. Why am I seeing poor results when running the PFG marker on my pulse field gel?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• N3019S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• N3019S_L_v1_0191401
• N3019S_L_v1_0191407
• N3019S_L_v1_0191504
• N3019S_L_v1_0191511
• N3019S_L_v1_0191604
• N3019S_L_v1_0191608
• N3019S_L_v1_0191612
• N3019S_L_v1_0191704
• N3019S_L_v1_0191709
• N3019S_L_v1_0201712
• N3019S_v1_10023487
• N3019L_v1_10023488
• N3019S_v1_10035838
• N3019S_v1_10058474
• N3019S_v1_10079761
• N3019S_v1_10100072
• N3019S_v1_10119265
• N3019S_v1_10144809
• N3019S_v1_10173181
• N3019S_v1_10211540
• N3019S_v1_10240199
• N3019S_v1_10262357

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• λ DNA-Mono Cut Mix
• Gel Loading Dye, Purple (6X), no SDS

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.