Product Class: Other

λ DNA-BstEII Digest

Product Introduction

  • Size range: 117 bp to 8,454 bp
  • Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)

 

Catalog # Size Concentration
N3014S 150 gel lanes (0.3 ml) 500 µg/ml

Product Information

Description

The BstEII digest of lambda DNA (cI857 ind 1 Sam 7) yields 14 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size.

Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.

  • Recommended gel percentage range: 0.6-1.2%
  • Optimum separation on 0.8% 

N3014_thumb
Lambda DNA-BstE II Digest visualized by ethidium bromide staining. 1.0% agarose gel. The full list of DNA fragments can be found in the chart below.
This product is related to the following categories:
DNA Markers & Ladders
This product can be used in the following applications:
DNA Analysis

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Bases

Fragment Mass (ng) bp
1 174 8,454
2 149 7,242
3 131 6,369
4 117 5,686
5 99 4,822
6 89 4,324
7 76 3,675
8 48 2,323
9 40 1,929
10 28 1,371
11 26 1,264
12 14 702
13 5 224
14 2 117

Effective Size Range

117bp to 8,454bp

Storage Buffer

10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C

Product Notes

  1. For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH20 and subsequently heated. Temperatures > 60°C may cause denaturation.
  2. The cohesive ends of fragments 1 and 4 may be separated by heating to 60°C for 3 minutes.
  3. 1X Gel Loading Dye, Purple, no SDS: 
    2.5% Ficoll®-400
    10mM EDTA
    3.3mM Tris-HCl (pH 8.0@25°C)
    0.02% Dye 1
    0.001% Dye 2

References

  1. Daniels, D.L. et al (1983). Appendix II:Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.
  2. Forster, A.C. et al. (1985). Nucl. Acids Res. 13, 745-761.

Protocols, Manuals & Usage

Protocols

  1. Suggested Loading Protocol for N3014 & N3012
  2. Suggested Loading Protocol for DNA Ladders & Markers

FAQs & Troubleshooting

FAQs

  1. What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
  2. How can I quantify the amount of DNA in each band of a marker?
  3. Why does my Lambda DNA molecular weight marker appear smeared and not represent the correct banding pattern?
  4. Can I use Midori Green with the DNA Ladders from NEB?
  5. Can I use SYBR® and/or GelRed® dyes with the DNA Ladders from NEB?
  6. Why is my DNA Ladder floating out the wells? Why are there no bands visible in my DNA ladder gel lane?

Tech Tips

Heat at 60C for 3 minutes to separate the cohesive ends of fragments 1 and 4.

To make it ready-to-load, dilute in TE buffer instead of water.

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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