Product Class: Other

NEBNext® Multiplex Oligos for MGI® (Dual Index Primer Pairs Set 1)

Product Introduction

NEBNext Multiplex Oligos for MGI (Dual Index Primer Pairs Set 1) enables high-yield, multiplexed MGI library production. The carefully selected pairs of index primers (following a combinatorial dual indexing scheme) are provided pre-mixed in a 96-well plate for ease of use. The small pack (NEB #E9725S) contains 24 pairs of index primers, while the large pack (NEB #E9725L) contains 96 pairs of index primers; the S and L packs may be combined for a total of 120 index primer pairs.

  • Convenient pre-selected pairs of primers for multiplex library prep for MGI
  • Can be combined for indexing of up to 120 samples
  • Single-use, pre-mixed wells ensure maximal purity with minimal cross contamination
Catalog # Size Concentration
E9725S 24 reactions
E9725L 96 reactions

Product Information

Description

The primer pairs in NEBNext Multiplex Oligos for MGI (Dual Index Primer Pairs Set 1) are used to incorporate index sequences (sample barcodes) into library molecules during PCR. The addition of these sample indices enables multiplexing; Set 1 allows for the multiplexing of up to 120 samples.

For sample sheets for use with MGI sequencers, scroll down to Usage Guidelines below "Protocols, Manuals & Usage".
This product is related to the following categories:
Library Preparation for MGI®,
Next Generation Sequencing Library Preparation,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • E9725S     -20    
      NEBNext® Dual Index Primer Pairs for MGI® E9648AVIAL -20 1 x 6 μl/well 20 µM
      NEBNext® Adaptor for MGI® E9650AVIAL -20 1 x 0.12 ml 40 µM
      NEBNext® Adaptor Dilution Buffer for MGI® E9651AVIAL -20 1 x 0.25 ml Not Applicable
  • E9725L     -20    
      NEBNext® Dual Index Primer Pairs for MGI® E9649AVIAL -20 1 x 6 μl/well 20 µM
      NEBNext® Adaptor for MGI® E9650AAVIAL -20 1 x 0.48 ml 40 µM
      NEBNext® Adaptor Dilution Buffer for MGI® E9651AAVIAL -20 1 x 1 ml Not Applicable

Properties & Usage

Protocols, Manuals & Usage

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Usage & Guidelines

FAQs & Troubleshooting

FAQs

  1. Can libraries be pooled before the circularization reaction when using the NEBNext® Circularization Module for MGI® (NEB #E9720)?
  2. Which library prep kits are compatible with the NEBNext® Multiplex Oligos for MGI® (Dual Index Primer Pairs Set 1) (NEB #E9725)?
  3. Which kit can I use for circularization to make single-strand, circular DNA libraries for DNBSEQ™ platforms?
  4. Are the primers in the NEBNext® Multiplex Oligos for MGI® (Dual Index Primer Pairs Set 1) (NEB #E9725) unique dual indices?
  5. How many NEBNext® index primer pairs are available for MGI® library prep?
  6. Can I use the 96-well primer plate to do my PCR reactions when using the NEBNext® Multiplex Oligos for MGI® (Dual Index Primer Pairs Set 1) (NEB #E9725)?
  7. How many primer reactions are in each well when using the NEBNext® Multiplex Oligos for MGI® (Dual Index Primer Pairs Set 1) (NEB #E9725)?
  8. How should the barcodes in the NEBNext® Oligos for MGI® (NEB #E9725) be sequenced?
  9. How should the barcodes be demultiplexed when using the NEBNext® Multiplex Oligos for MGI® (Dual Index Primer Pairs Set 1) (NEB #E9725)?
  10. What are the adaptor sequences for trimming when using the NEBNext® Multiplex Oligos for MGI® (Dual Index Primer Pairs Set 1) (NEB #E9725)?
  11. Where can I find the sample sheets for these Multiplex Oligos when using the NEBNext® Multiplex Oligos for MGI® (Dual Index Primer Pairs Set 1) (NEB #E9725)?
  12. The ligation reaction is very viscous. What will happen if it is not mixed properly?
  13. What is the concentration of the adaptor and primers?
  14. Do the NEBNext® library prep kits for MGI® include adaptors and primers?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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