Product Class: Other

NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing


Catalog #E7672

Product Introduction

This new module contains the NEBNext DNA repair, end repair and ligation reagents recommended for Oxford Nanopore Technologies updated SQK-LSK114 singleplex ligation sequencing library prep protocols.

  • Includes Salt-T4® DNA Ligase & NEBNext FFPE DNA Repair Buffer v2
  • Component volumes tailored for use with many SQK-LSK114 workflows
  • Simplified ordering and inventory management
  • Compatible with all devices: MinION®, GridION®, PromethION®, Flongle®
  • No waste – no unnecessary buffers or excess reagents

Product Information

Description

This module contains the following reagents, at volumes designed for use with Oxford Nanopore Technologies updated SQK-LSK114 singleplex ligation sequencing library prep protocols.

  • NEBNext FFPE DNA Repair Mix
  • NEBNext FFPE DNA Repair Buffer v2
  • NEBNext Ultra™ II End Prep Enzyme Mix
  • Salt-T4 DNA Ligase

The appropriate device-specific SQK-LSK114 protocol from Oxford Nanopore Technologies should be followed for use of these reagents.

This product is related to the following categories:
Library Preparation for Oxford Nanopore Technologies,
Next Generation Sequencing Library Preparation,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • E7672S     -20    
      NEBNext® FFPE DNA Repair Buffer v2 E7673AVIAL -20 1 x 0.168 ml Not Applicable
      NEBNext® FFPE DNA Repair Mix E7182AVIAL -20 1 x 0.048 ml Not Applicable
      NEBNext® Ultra™ II End Prep Enzyme Mix E7184AVIAL -20 1 x 0.072 ml Not Applicable
      Salt-T4® DNA Ligase E7674AVIAL -20 1 x 0.24 ml Not Applicable
  • E7672L     -20    
      NEBNext® FFPE DNA Repair Buffer v2 E7673AAVIAL -20 1 x 0.672 ml Not Applicable
      NEBNext® FFPE DNA Repair Mix E7182AAVIAL -20 1 x 0.192 ml Not Applicable
      NEBNext® Ultra™ II End Prep Enzyme Mix E7184AAVIAL -20 1 x 0.288 ml Not Applicable
      Salt-T4® DNA Ligase E7674AAVIAL -20 1 x 0.96 ml Not Applicable

Properties & Usage

Protocols, Manuals & Usage

Usage & Guidelines

FAQs & Troubleshooting

FAQs

  1. What is the difference between NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB #E7672) & NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (NEB #E7180)?
  2. Is the NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing the best choice if I am performing native barcoding ONT sequencing?
  3. Which protocol should be followed when using the NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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