NEBNext® Ultra™ II Ligation ModuleProduct information
NEBNext Ultra II Ligation Module
|24 rxns||-||Unavailable in your region|
NEBNext Ultra II Ligation Module
|96 rxns||-||Unavailable in your region|
This module is part of the Ultra™ II workflow, and is optimized for use with the NEBNext® Ultra II End Repair/dA-Tailing Module (NEB #E7546), for Illumina®-compatible library construction.
This module is also compatible with some Oxford Nanopore Technologies® workflows, including some involving COVID-19 research.
The NEBNext Ultra II Ligation Module has been optimized to ligate efficiently DNA adaptors compatible with Illumina sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546), and is part of the Ultra II DNA workflow which enables high yield preparation of high quality libraries from 500 pg to 1 µg of input DNA.
This module is compatible with Illumina workflows and with several Oxford Nanopore Technologies workflows.
For Illumina library construction, the NEBNext Ultra II Ligation Module is designed for use with the following:
- NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546)
- NEBNext Ultra II Q5® Master Mix (NEB #M0544)
- NEBNext Oligos for Illumina (NEB #E7335, #E7500, #E7710, #E7730, #E6609, #E7600 #E7535, NEB #E7350)
- This product is related to the following categories:
- Library Preparation for Illumina,
- DNA Library Prep for Illumina,
- RNA Library Prep for Illumina,
- This product can be used in the following applications:
- NEBNext® Ultra™ II DNA Library Prep
The following reagents are supplied with this product:
|NEB #||Component Name||Component #||Stored at (°C)||Amount||Concentration|
Properties & Usage
Materials Required but not SuppliedMagnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S) or Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)
Multiplex Oligos (Neb.com/oligos)
NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546) or NEBNext Ultra II FS DNA Module (NEB #E7810) and associated reagents and consumables.
NEBNext Ultra II Q5® Master Mix (NEB #M0544)
Protocols, Manuals & Usage
FAQs & Troubleshooting
Citations & Technical Literature
- Oxford Nanopore Technologies (2020) PCR Tiling of COVID-19 Virus Oxford Nanopore Technologies Community; Protocol version: PTC_9096_V109_REVF_06FEB2020;
- Resende, P. (2020) Long reads nanopore sequencing to recover SARS-CoV-2 whole genome V.3 protocols.io; DOI: dx.doi.org/10.17504/protocols.io.bfy7jpzn
- Quick, J. (2020) nCoV-2019 sequencing protocol v2 protocols.io; DOI: dx.doi.org/10.17504/protocols.io.bdp7i5rn
Quality, Safety & Legal
Quality Assurance StatementQuality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
SpecificationsThe Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate Of AnalysisThe Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety DataSheetsThe following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
NEBNext® Ligation Enhancer
NEBNext® Ultra™ II Ligation Master Mix
Legal and DisclaimersProducts and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email email@example.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
The supporting documents available for this product can be downloaded below.