Product Class: Kit

LunaScript® RT Master Mix Kit (Primer-free)


Catalog #E3025

Product Introduction

The LunaScript RT Master Mix Kit (Primer-free) features an optimized 5X master mix containing all the necessary components for first strand cDNA synthesis, except primers.

  • 5X master mix contains Luna® Reverse Transcriptase, dNTPs, and Murine RNase Inhibitor
  • Primer-free format enables user flexibility for choice of primers for optimal cDNA synthesis
  • Complete first strand cDNA synthesis protocol in less than 15 minutes
  • Can be used for first strand cDNA synthesis, two-step RT-qPCR, two-step RT-PCR, and RNA-seq workflows
  • Non-interfering, visible tracking dye helps to eliminate pipetting errors
  • Includes No-RT control mix for increased confidence
 

Protocols, Manuals & Usage

Protocols

  1. LunaScript RT Master Mix Kit Protocols (NEB #E3025)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

FAQs & Troubleshooting

FAQs

  1. Can I use  RNA samples purified from different storage conditions such as RNALater in my LunaScript®  cDNA synthesis reactions?
  2. Can I set up my cDNA synthesis reaction at room temperature when using the LunaScript®  RT SuperMix?
  3. Can I use LunaScript® cDNA products directly in qPCR? How much can I use for detection in a qPCR reaction?
  4. How much RNA template should I use in my LunaScript®  cDNA synthesis reactions?
  5. How should I store LunaScript®  cDNA products?
  6. How should I choose between a one-step RT-qPCR or two-step RT-qPCR approach?
  7. Should I include a No-Reverse Transcriptase (RT) control reaction when setting up my cDNA synthesis reaction?
  8. What qPCR reagents do you recommend for detection of cDNA products?
  9. Why do I have low cDNA yields?
  10. Will the blue dye in the LunaScript®  RT SuperMix interfere with detection?
  11. How do I choose between the first strand cDNA synthesis kits from NEB?
  12. When using LunaScript cDNA products in downstream PCR (i.e., two-step RT-PCR), what volumes and PCR products can be used? 
  13. How do I choose primers for cDNA synthesis using LunaScript RT Master Mix (Primer-free)?
  14. Can I use LunaScript RT Master Mix in RNA-seq workflows?
  15. What temperature should I use for my LunaScript®  cDNA synthesis reaction?
  16. How do I choose between Induro Reverse Transcriptase and the different LunaScript and ProtoScript products?
  17. How do I know whether my template RNA is of good quality?

Troubleshooting

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