NucleoBond RS 50 Column for purification of vaccine-grade plasmid DNA

MACHEREY-NAGEL offers a range of ultra-fast and simple-to-use plasmid DNA purification kits that provide consistent results in downstream applications including transfection, cloning, sequencing, PCR, transformation, and restriction analysis. These kits are available in miniprep, midiprep, maxiprep, megaprep, gigaprep, and/or high-throughput formats using silica membrane-based NucleoSpin technology or anion exchange-based NucleoBond technology. Our kits are available in transfection-grade or endotoxin-free for more demanding applications.

Column for preparative scale isolation of vaccine-grade plasmid DNA with peristaltic pump or FPLC system

Application	Vaccine-grade plasmid DNA isolation
Target	Plasmid DNA
CE certified	No, research use only
Technology	Anion exchange chromatography
Brand	NucleoBond
Format	Preparative scale
Handling	Peristaltic pump, FPLC
Lysate clarification	Bottle top filter, Filter cartridges
Automated use	No
Sample material	Bacteria, E. coli
Sample amount	Maximum 40 g wet weight
Vector size	< 300 kbp
Theoretical binding capacity	Up to 50 mg
Typical purity A260/A280	1.8–2.0
Endotoxin level	≤ 0.01 EU/µg DNA
Shelf life (from production)	24 Month(s)
Scope of delivery	1x NucleoBond RS 50 Column
Hazardous material	No

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