Principle / Procedure

NucleoSpin® 8 / 96 Plant II kits are designed for the isolation of genomic DNA from plant tissue (e.g., arabidopsis, tobacco, or maize). Depending on the individual sample (20–100 mg) typical yields are in the range from 5 up to 30 μg DNA. For RNA-rich samples, we recommend to add RNase to the lysis buffer or perform a subsequent RNase treatment. The general isolation procedure ensures lysis of plant material with a CTAB lysis buffer developed especially for plant samples. CTAB is removed completely in the subsequent washing steps and thus does not interfere with downstream PCR. The binding process is reversible and specific for nucleic acids. Contaminations are removed by washing. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer. The obtained DNA can be used directly for PCR, Southern blotting, or any kind of enzymatic reaction. NucleoSpin® 8 / 96 Plant II can be used in a centrifuge and under vacuum, manually and automated on many common liquid handling instruments.

Required hardware

Centrifugation
For centrifuge processing of NucleoSpin® 8 / 96 Plant II kits a microtiterplate centrifuge is required which is able to accommodate the NucleoSpin® Plant II Strips / Plate stacked on a Round- or Square-well Block or Rack with Tube Strips and reaches accelerations of 5,600–6,000 x g (bucket height: 85 mm). For further information on suitable centrifuges please contact our Technical Service.
For centrifuge processing of the 8-well Strips the reusable Starter Set C, containing Column Holders C, NucleoSpin® Dummy Strips, MN Square-well Blocks, Tube Strips is required.

Vacuum processing
The NucleoSpin® 8 / 96 Plant II kits can be used manually with the NucleoVac 96 Vacuum Manifold. Additionally, a suitable centrifuge for sample preparation steps may be required.
For vacuum processing of the 8-well Strips the reusable Starter Set A, containing Column Holders A and Dummy Strips is required. For automation on laboratory platforms with standard 96-well plate vacuum chambers the use of the Starter Set A is also required.

Features

Isolation of genomic DNA from plant cells and tissue in flexible 8-well strip format and for high throughput in approved 96-well format

• Time-saving parallel isolation of genomic DNA from plant cells and tissue
• Processing possible under vacuum* or by centrifugation
• Suitable for manual and automated processing
• Innovative MN Wash Plate minimizes risk of cross-contamination
• DNA ready to use for any kind of enzymatic reaction
• NucleoSpin® 8 / 96 Plant II Core Kit: Kits with basic content focussed on automation platforms.
  Additional accessories can be combined as needed.

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