The RNase A is primarily intended to be used with NucleoSpin® and NucleoBond® kits. See individual protocols for detailed information. However, if the RNase A is used for another than the recommended purpose performance may differ considerably from its expected one.
Nucleotides and small oligonucleotides are removed by the gentle binding conditions and the stringent washing steps. To remove contamination of RNA completely, it is recommend to add 1 µg of RNase A to 150 µL of sample and to incubate at room temperature (18–25 °C) for 5–15 min.
Specification
Source: Bovine pancreas
Form: 100 mg / or 50 mg white freeze-dried material
RNase activity: 120 U/mg
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The supporting documents available for this product can be downloaded below.