Principle

Separation of the precipitate can be performed by centrifugation (NucleoBond® Xtra Midi EF or Maxi EF) or by using the NucleoBond® Finalizer/ Finalizer Large included in the NucleoBond® Xtra Midi Plus EF/Maxi Plus EF kits to reduce the total prep time to about 45 minutes (Midi Plus EF) or 50 minutes (Maxi Plus EF).

Data NucleoBond® Xtra Maxi Plus EF

Comparison of transfection efficiencies using plasmid DNA purified with NucleoBond® Xtra Maxi Plus EF and two competitor products
Plasmid purification: 4.5 kbp high-copy plasmid, bacterial strain: E. coli DH5a®, purification according to each manufacturer’s protocol using 500 ml LB overnight culture with high plasmid content
Plasmid yields: NucleoBond® Xtra Maxi Plus EF: 1240 µg, competitor A (EF kit): 650 µg, competitor B (EF kit): 250 µg
Transfection experiment: cells transfected in 24-well plates, 7.5 x 104 cells/well, transfection with 1 µg plasmid DNA/well, transfection reagent: DreamFect™ Gold (OZ Biosciences), results shown after 24 hours
Besides increased yields and highly reduced preparation times, plasmid DNA purified with NucleoBond® Xtra Maxi Plus EF shows comparable or even higher transfection efficiencies than DNA prepared with both competitor kits

Data kindly provided by NEB - OZ Biosciences, Marseille, France, www.ozbiosciences.com

Specification

NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizer, buffers, RNase A #740422.10

NucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A #740424.10

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