Binding Buffer NTI with pH indicator

The optimal pH to bind even small DNA fragments to the silica membrane is about 5–6. Binding Buffer NTI is sufficiently buffered to maintain this pH. However, to be sure that the pH is right even for samples with extreme alkaline pH or high buffer concentration, a pH indicator has been added. The pH indicator does not interfere with DNA binding and is completely removed during the purification. The yellow color is also beneficial for gel extractions,making it easy to identify undissolved pieces of agarose.
For restoring the correct conditions add more Buffer NTI or 4 M sodium acetate (NaAc, pH 5.0), or small amounts of hydrochloric acid (HCl) until the color switches back to yellow.

Click below to order the optional binding buffers:

NucleoSpin buffer NTB NucleoSpin bufer NTC

For more detailed information and documents, click here.