Principle

Plant and fungal material is diverse in terms of structure, cell wall composition, biochemical compounds and the types of organs. Common products for the RNA isolation from plant material usually work fine with samples such as Arabidopsis leaves, but fail to efficiently purify RNA from challenging samples. The NucleoSpin® RNA Plant and Fungi kit was developed to efficiently purify high integrity RNA from samples rich in secondary metabolites, sugar, starch or other compounds that interfere with nucleic acid purification methods. The kit enables RNA isolation from diverse plant organs like seeds, roots, stems, needles and others. Of course, it can also be used for standard samples such as Arabidopsis leaves.

 

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Technology	Silica membrane technology
Format	Mini spin column
Sample material	< 500 mg plant / fungal material
Fragment size	> 200 nt
Typical yield	20-70 µg
A260/A280	1.9–2.1
A260/A230	~ 2
Typical RIN (RNA integrity number)	7-9
Elution volume	50 µL
Preparation time	25 min / 6 preps
Binding capacity	200 µg

Successfully tested sample types

This table shows a selection of samples that were successfully tested with NucleoSpin® RNA Plant and Fungi.

Applications*

• RNA isolation from plant and fungi
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

* Kits to be used for research purposes only

 

 

NucleoSpin® RNA Plant and Fungi – RNA extraction data

The NucleoSpin® RNA Plant and Fungi kit enables efficient isolation of high integrity RNA from various sample types. After RNA isolation with NucleoSpin® RNA Plant and Fungi (MN) and two competitor products (S, Q), the RNA was analyzed and quantified with an Agilent Bioanalyzer.
(A) RNA isolation from 500 mg kiwi fruit
(B) RNA isolation from 50 mg potato tuber
(C) RNA isolation from 50 mg spruce needles

The NucleoSpin® RNA Plant and Fungi kit allows for extraction of high integrity RNA from fungal samples. The RNA was isolated from 3 independent samples of fungal fruiting body and fungal mycelium. Analysis of the RNA was performed with an Agilent Bioanalyzer
(D) RNA isolation from 50 mg Agaricus campestris fruiting body
(E) RNA isolation from 100 mg Botrytis cinerea mycelium

 

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