Principle / Procedure

The isolation of genomic DNA (gDNA) from the majority of organs is a challenging task.  Organs are composed of different tissues with various types of cells and matrices.  Large molecules such as collagens and proteoglycans can impede lysis and even impair downstream enzymatic reactions (e.g., PCR). The new NucleoSpin® 96 DNA RapidLyse kit uses a unique lysis chemistry to release gDNA from organs and cells with the highest efficiency.

Applications*

Manual or automated isolation of DNA from human and animal tissue, cultured cells, or bacteria.

* Kits to be used for research purposes only.

Highest DNA Yields

Different mammalian organs were lysed and DNA was extracted. Same amounts of individual eluates were subjected to gel electrophoresis. Highest yields of DNA could be extracted with NucleoSpin^® 96 DNA RapidLyse. Q represents a competitor kit, MN represents NucleoSpin^® 96 DNA RapidLyse.

Best qPCR performance

DNA was isolated with NucleoSpin® 96 DNA RapidLyse (orange) or with a kit from Competitor Q (blue). A 136 bp fragment of the 18S rRNA gene was amplified by qPCR. Lowest Ct-values were detected when DNA was isolated with NucleoSpin® 96 DNA RapidLyse, indicating highest gDNA yields.

Cost efficient HTP solution

The average prep price of NucleoSpin® DNA 96 RapidLyse was compared to prep prices for other high-throughput genomic DNA purification products. The NucleoSpin® 96 DNA RapidLyse is the most cost-efficient product for gDNA isolation compared to competitors R, Q, T, Z, and S. MN represents NucleoSpin® 96 DNA RapidLyse.

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