Protino Ni-IDA Resin
Product informationBenefits
Protino® Ni-IDA, the purer alternative for higher yields of His-tag proteins:
- Chelating group IDA (iminodiacetic acid), three protein binding sites (also see Technologies)
- High protein yield / recovery even from diluted samples
- High protein concentration
- High binding specificity, less unspecific binding of contaminating proteins compared to other common IMAC matrices
- Dry resin → storage at room temperature
Protino® Ni-IDA Resin:
- Dry bulk material
- Rigid support silica matrix, compatible with high pressure and high flow rates, does not shrink or swell → highest flexibility
- Batch procedure, gravity-flow column chromatography, FPLC™ applications
- Empty Protino® columns available for use with Protino® Ni-IDA Resin
- Empty 96-well Receiver Plates available for use with Protino® Ni-IDA Resin
| Code | Name | Size | Quantity | Price | |
|---|---|---|---|---|---|
745210.5 |
Protino Ni-IDA Resin |
5 g | - | Unavailable in your region | |
745210.30 |
Protino Ni-IDA Resin |
30 g | - | Unavailable in your region | |
745210.120 |
Protino Ni-IDA Resin |
120 g | - | Unavailable in your region | |
745210.600 |
Protino Ni-IDA Resin |
600 g | - | Unavailable in your region |
Principle
Protino® Ni-IDA products enable fast and convenient purification of recombinant polyhistidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC). Protino® Ni-IDA is a dry silica-based resin precharged with Ni2+ ions. Binding of protein is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions. The chelating group of Protino® Ni-IDA is based on IDA (iminodiacetic acid), which enables strong and efficient binding of target protein onto the IMAC matrix. In contrast to traditional IDA matrices, Protino® Ni-IDA shows an optimized, low density of IDA ligands, which is created by a special manufacturing process. This non-saturating surface concentration of IDA eliminates non-specific interactions of contaminating proteins with the adsorbent. As a result, Protino® Ni-IDA ensures higher target protein purity.
IDA is a threedentate chelator which occupies three of the six binding sites in the coordination sphere of the Ni2+ ion. The remaining three coordination sites are usually occupied by water molecules and can be exchanged with histidine residues of the recombinant protein.
Features
| Technology | IMAC, immobilized metal ion affinity chromatography |
| Chelating ligand | IDA (iminodiacetic acid) |
| Format | Bulk matrix |
| Matrix | Macroporous silica |
| Physical form | Dry matrix, precharged with Ni2+ |
| Binding capacity* | 20 mg/g resin 10 mg/ml bed volume |
| Max. pressure | 145 psi (10 bar) |
* Binding capacity will vary for each polyhistidin-tagged protein
For more detailed information and documents, click here.
The supporting documents available for this product can be downloaded below.