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NucleoSpin RNA Midi

Product information

Benefits

  • Efficient removal of genomic DNA – rDNase included for on-column digestion*
  • Efficient sample homogenization and reduction of viscosity – NucleoSpin® Filters L (shredders) included
  • Up to 600 μg ready-to-use RNA

* The amount of DNA contamination is significantly reduced during on-column rDNase digestion. Anyhow, in very sensitive applications it might be possible to detect traces of DNA. The NucleoSpin RNA Midi system is checked by the following procedure: One million HeLa cells are subjected to RNA isolation according to the protocol. RNA eluate is used as template for PCR detection of a 1 kb fragment in a 30 cycle reaction. Generally, no PCR fragment is obtained if the rDNase is applied. However, a strong PCR fragment is obtained if rDNase is omitted. The eventuality of DNA detection with PCR increases with:
– the number of DNA copies per preparation: single copy target < plastidial/ mitochondrial target < cells transfected with plasmid
– decreasing PCR amplicon size

Code Name Size Quantity Price
740962.20
NucleoSpin RNA Midi (20)
20 preps    - Unavailable in your region
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