Product Class: Kit

Luna® Universal One-Step RT-qPCR Kit

Product Introduction

Rapid, sensitive and precise dye-based qPCR detection and quantitation of target RNA targets

Make a simpler choice

  • One product per application simplifies selection
  • Convenient master mix formats and user-friendly protocols simplify reaction setup
  • Non-interfering, visible tracking dye helps to eliminate pipetting errors

Experience best-in-class performance

  • All Luna® products have undergone rigorous testing to optimize specificity, sensitivity, accuracy and reproducibility
  • Products perform consistently across a wide variety of sample sources
  • A comprehensive evaluation of commercially-available qPCR and RT-qPCR reagents demonstrates superior performance of Luna products

Optimize your RT-qPCR with Luna WarmStart® Reverse Transcriptase

  • Novel, thermostable reverse transcriptase (RT) improves performance
  • WarmStart RT paired with Hot Start Taq increases reaction specificity and robustness
Catalog # Size Concentration
E3005S 200.0 reactions
E3005L 500.0 reactions
E3005X 1000.0 reactions
E3005E 2500.0 reactions

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Protocols, Manuals & Usage

Protocols

  1. Luna® Universal One-Step RT-qPCR Kit Protocol (E3005)

Manuals

The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

Usage & Guidelines

Tools & Resources

Web Tools

FAQs & Troubleshooting

FAQs

  1. How do I use qPCR to determine the concentration of my material?
  2. Can I set up my Luna® RT-qPCR at room temperature?
  3. What is the difference between probe- and dye-based versions of the Luna® qPCR Mixes?
  4. Should I use probe- or dye-based detection for my qPCR assays?
  5. How should I design primers for Luna® qPCR?
  6. How long should my amplicon be for qPCR?
  7. Why is the Luna® qPCR Mix blue? Will this dye interfere with detection?
  8. Can I run the Luna® qPCR Mix on my qPCR instrument?
  9. Can I use fast instrument settings with the Luna® qPCR Mix?
  10. Do I need to add ROX?
  11. How many dilutions should I use to make a standard curve?
  12. Why does NEB recommend 40-45 cycles?
  13. Does the Luna® qPCR Mix contain dUTP? Can I use carryover contamination prevention methods?
  14. Why do I have multiple peaks in my melt curve?
  15. How can I distinguish non-template amplification (NTC) from real products?
  16. Why do I see amplification curves in my NTC samples?
  17. How much primer should I use with the Luna® Universal RT-qPCR Kit?
  18. How do I choose between one-step RT-qPCR and two-step RT-qPCR?
  19. What RNA samples can be used in RT-qPCR with the Luna® Mix?
  20. How much RNA template should I use in my RT-qPCR reaction?
  21. Can I use longer targets in one-step RT-qPCR?
  22. What temperature should I use for cDNA synthesis with Luna® RT-qPCR kits?
  23. Should I include a no Luna® WarmStart® Enyzme Mix control (-RT Control)?
  24. What is the fluorescent, double-stranded DNA binding dye in the Luna® qPCR master mix?
  25. Is the Monarch® Total RNA Miniprep Kit compatible with Luna® RT-qPCR Reagents?
  26. Are the Monarch RNA Cleanup Kits compatible with Luna RT-qPCR reagents?
  27. Can I use shorter cycling times?

Troubleshooting

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