Product Class: Other

Sodium Orthovanadate (Vanadate)

Vanadate has been fully activated.

Product Introduction

Sodium Orthovanadate (Vanadate) is a general competitive inhibitor for protein phosphotyrosyl phosphatases. The inhibition by Sodium Orthovanadate is reversible upon the addition of EDTA or by dilution.

  • Can be used to preserve the protein tyrosyl phosphorylation state in cells, cell lysates, and protein tyrosine kinase assays
Catalog # Size Concentration
P0758S 1 ml 100 mM
P0758L 5 ml 100 mM

Product Information

Description

Sodium Orthovanadate (Vanadate, Na3VO4) is a commonly used general inhibitor for protein phosphotyrosyl phosphatases (PTPs). It is a competitive inhibitor. The inhibition by Vanadate is completely reversible upon the addition of EDTA or by dilution. Vanadate has been activated for maximal inhibition of PTPs following the procedure described by J.A. Gordon (1).

The PTP activities are conveniently separable from the protein phosphoseryl and phosphothreonyl phosphatase (PSP) activities inhibited by Fluoride and EDTA. Routinely Vanadate is used to preserve the protein tyrosyl phosphorylation state in cells, cell lysates, and protein tyrosine kinase assays (1, 2). 
This product is related to the following categories:
Protein Kinases,
Protein Phosphatases,
This product can be used in the following applications:
Protein Phosphatases and Kinases

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Product Notes

  1. Common buffer components such as EDTA and reducing agents may interact with Vanadate, affecting its potency (2).
  2. Storage: Sterile purified water adjusted to pH 10.0 (1,2).

References

  1. Gordon, J.A. (1991). Methods in Enzymology. 201, 477-482.
  2. Huyer, G. et al. (1997). J. Biol. Chem.. 272, 843-851.

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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