pUC19 VectorProduct information
|50 µg ( 1000 µg/ml )||-||Unavailable in your region|
|250 µg ( 1000 µg/ml )||-||Unavailable in your region|
- View sequence details
- pUC19 is a commonly used cloning vector that conveys the Amp resistance.
- The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number.
- pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1).
- NEB offers a selection of common cloning plasmids and DNAs for use as substrates.
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Traditional Cloning Workflow
DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.
Product SourcepUC19 is isolated from E. coli ER2272 (dam+ dcm+ EcoK M-) by a standard plasmid purification procedure.
Polylinker DNA Sequence
GENBANK Assession NumberL09137
- This product is related to the following categories:
The following reagents are supplied with this product:
|NEB #||Component Name||Component #||Stored at (°C)||Amount||Concentration|
Properties & Usage
Copy NumberHigh (100+)
Origin of Replication
- pMB1 (high-copy mutant)
Lac Repressor on Plasmid0
10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C
- Yanisch-Perron, C., Vieira, J. and Messing, J. (1985). Gene. 33, 103-119.
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