Product Class: Other

dUTP Solution


Catalog #N0459

Product Introduction

  • ≥ 99% purity as determined by HPLC analysis
  • For use with polymerases such as Taq DNA Polymerase (NEB# M0273) or Q5U® Hot Start High-Fidelity DNA Polymerase (NEB# M0515)
  • Combine with Antarctic Thermolabile UDG (NEB# M0372) to prevent carryover contamination in PCR and qPCR-related technologies
 

Product Information

Description

Contains 0.25 ml of 100 mM ultrapure dUTP. dUTP can either be fully substituted for dTTP or added in addition to conventional dNTPs. dUTP can be used with Family A polymerases (such as Taq) or modified Family B polymerases, such as Q5U Hot Start High-Fidelity DNA Polymerase. Q5U contains a mutation in the uracil-binding pocket that enables the ability to read and amplify templates containing uracil and inosine bases.

For a simplified protocol, dUTP can be added to most polymerase reactions at the same concentration as other nucleotides (e.g., a 1:1:1:1:1 dA/dC/dG/dT/dU ratio). 

For applications that require other molar ratios (up to and including full substitution of dU for dT), custom nucleotide mixes must be made. We recommend starting with a concentration of 0.2 mM of each dNTP for both Taq and Q5U DNA polymerases.

Supplied in: 
Ultra purified water as a sodium salt at pH 7.5.

Concentration: 
The dUTP Solution is supplied at a concentration of 100 mM (+/- 5 mM) as determined by A260 absorbance.

Diluent Compatibility: 
Can be diluted using sterile distilled water, preferably Milli-Q® water or can be diluted using sterile TE [10 mM Tris-HCl, 1 mM EDTA (pH 7.5)].

 
This product is related to the following categories:
Nucleotide Solutions,
PCR, qPCR & Amplification Technologies,
This product can be used in the following applications:
USER® Cloning,
Applications of USER® and Thermolabile USER II Enzymes,
High-Fidelity PCR,
DNA Amplification, PCR & qPCR,
Specialty PCR,
Routine PCR,
PCR

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Certificate Of Analysis

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The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information about commercial rights, please email us atgbd@neb.com While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications