Product Class: Other

Pyrophosphatase, Inorganic (yeast)
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Developed by the Biohelix Corporation, and produced at New England Biolabs.

Product Introduction

  • Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate
  • Enhances yields of RNA in transcription reactions
  • Isolated from a recombinant source
Catalog # Size Concentration
M2403S 10 units 100 units/ml
M2403L 50 units 100 units/ml

Product Information

Description

Highlights

  • Isolated from a recombinant source.
Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate.

A variety of metabolic reactions generate inorganic pyrophosphate as a reaction byproduct. Such reactions are rendered irreversible when the pyrophosphate is degraded by pyrophosphatase (1). RNA and DNA synthesis are examples of reactions that can be pulled far in the synthesis direction by the action of inorganic pyrophosphatase.

Product Source

PPase is prepared from an E. coli strain containing a genetic fusion of the Saccharomyces cerevisiae ppa gene and the gene coding for Mycobacterium xenopi GyrA intein. The final product has been cleaved and purified from the intein fusion partner. Developed by the Biohelix Corporation, and produced at New England Biolabs.
This product is related to the following categories:
Phosphatases,
RNA Modification,
RNA Synthesis In vitro Transcription (IVT),
Nucleotide Solutions for RNA,
This product can be used in the following applications:
Whole Genome Amplification & Multiple Displacement Amplification

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

One unit is the amount of enzyme that will generate 1 µmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25°C in 100 mM Tris-HCl, [pH 7.2], 2 mM MgCl2 and 2 mM PPi in a reaction volume of 0.5 ml).

Storage Buffer

20 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 8 @ 25°C

Molecular Weight

Theoretical: 71 kDa

Application Features

  • Enhancing yields of RNA in transcription reactions (2)

Product Notes

  1. While a specific NEBuffer is not supplied, Yeast Inorganic Pyrophosphatase works well in any buffer containing Mg++ (1-10 mM). This enzyme is typically used in RNA IVT reactions, where the Mg++ concentration falls within this range.

References

  1. Kornberg, A. (1980). DNAReplication. 54-55, 94. W.H. Freeman and Company..
  2. Cunningham, P.R. and Ofengand, J. (1990). Biotechniques. 9, 713-714.
  3. Cooperman, B.S. (1982). MethodsEnzymol.. 87 (pt. C), 526-548.

FAQs & Troubleshooting

FAQs

  1. Can alternate metals be used as activators for Yeast Inorganic Pyrophosphatase?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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