Sce PUS1
Product informationSce PUS1
We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.
Catalog #M0526
- Sce PUS1 isomerizes uridine to pseudouridine in predominantly single-stranded RNA in vitro
- Sce PUS1 is a stand-alone enzyme and does not require an RNA guide or co-factor to modify RNA
- Sequence-specific pseudouridine modification by Sce PUS1 is an alternative to randomly incorporated modified nucleosides by RNA polymerases
This is an Enzyme for Innovation (EFI). EFI is a project initiated by NEB to provide unique enzymes to the scientific community in the hopes of enabling the discovery of new and innovative applications. These enzymes have interesting properties and unique specificities.

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Product Information
Sce Pseudouridine Synthase I (Sce PUS1) converts Uridine to Pseudouridine in single-stranded RNA, with a preference for Uridines in single-stranded RNA regions over Uridines in double-stranded RNA. The optimal substrate is an unstructured RNA that is 15 nt long or longer.
- This product is related to the following categories:
- Epitranscriptome Analysis,
- RNA Modification
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Protocols, Manuals & Usage
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FAQs & Troubleshooting
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Quality, Safety & Legal
The supporting documents available for this product can be downloaded below.