Companion Products

• T7 RNA Polymerase
• RNase Inhibitor, Murine
• Proteinase K, Molecular Biology Grade
• Monarch^® Spin RNA Cleanup Kit (10 µg)
• Monarch^® RNA Cleanup Kit (50 µg)
• Monarch^® Spin RNA Cleanup Kit (500 µg)
• RNA Loading Dye, (2X)
• Nucleoside Digestion Mix

1. Arluison, V., Buckle, M., & Grosjean, H. (1999). Pseudouridine synthetase Pus1 of Saccharomyces cerevisiae: kinetic characterisation, tRNA structural requirement and real-time analysis of its complex with tRNA.  J Mol Bio. 289 (3), 491-502.
2. Arluison, V., Batelier, G., Riès-Kautt, M., & Grosjean, H. (1999). RNA:pseudouridine synthetase Pus1 from Saccharomyces cerevisiae: oligomerization property and stoichiometry of the complex with yeast tRNA (Phe). Biochimie. 81 (7), 751-756.
3. Carlile, T. M., Martinez, N. M., Schaening, C., Su, A., Bell, T. A., Zinshteyn, B., & Gilbert, W. V (2019). mRNA structure determines modification by pseudouridine synthase 1. Nature Chemical Biolog. 15 (10), 966-974.
4. (2014). Pseudouridine profiling reveals regulated mRNA pseudouridylation in yeast and human cells. Nature. 515 (725), 143-146.
5. (1998). The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity.. 4 (7), 856-869.
6. Grünberg S, Doyle LA, Wolf EJ, Dai N, Corrêa IR Jr, Yigit E, et al (2023). The structural basis of mRNA recognition and binding by yeast pseudouridine synthase PUS1. 18(11), DOI: e0291267, PLoS ONE.

1. Protocol for conversion of Uridine to Pseudouridine using Sce PUS1 (NEB #M0526)

1. What is the effect of Mg²⁺ concentration on Sce PUS1 activity?
2. How can Sce PUS1 activity be improved when low concentrations of RNA are used?
3. What effect does MnCl₂ have on Sce PUS1 activity?
4. What is the effect of NaCl concentration on Sce PUS1 activity?
5. Can Sce PUS1 modify more than 1 µg of RNA in one reaction?
6. Can I scale up or scale down the reaction volume of the Sce PUS1 reaction?
7. Can I shorten or extend the incubation time of Sce PUS1 with target RNA?
8. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0526S_v1_10078665
• M0526S_v1_10091724
• M0526S_v1_10121361
• M0526S_v1_10145561
• M0526S_v1_10184218
• M0526S_v1_10205439
• M0526S_v1_10232693
• M0526S_v1_10249251

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Sce PUS1

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.