The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0492S     -20       Q5® High-Fidelity 2X Master Mix M0492SVIAL -20 2 x 1.25 ml 2 X
M0492L     -20       Q5® High-Fidelity 2X Master Mix M0492SVIAL -20 10 x 1.25 ml 2 X

Application Features

• High-fidelity PCR
• Cloning
• Long or Difficult Amplification
• High-throughput PCR

Companion Products

• Deoxynucleotide (dNTP) Solution Mix
• Deoxynucleotide (dNTP) Solution Set
• Q5® High-Fidelity DNA Polymerase
• Q5® Hot Start High-Fidelity 2X Master Mix
• Q5® Hot Start High-Fidelity DNA Polymerase
• Q5® Reaction Buffer Pack

1. A precipitate (most noticeable after the first 1–2 freeze/thaw cycles) is not uncommon. To ensure optimal performance, the master mix should be thawed and resuspended prior to use. Stability testing using up to 15 freeze/thaw cycles has shown no negative effect on master mix performance.
   
   
2. Product specifications for individual components in the Q5 High-Fidelity 2X Master Mix are available separately.

1. Anastassia Voronova Erin Coyne, Ashraf Al Madhoun, Joel V. Fair, Neven Bosiljcic, Catherine St-Louis, Grace Li, Sherry Thurig, Valerie A. Wallace, Nadine Wiper-Bergeron, and Ilona S. Skerjanc. (2013). Hedgehog Signaling Regulates MyoD Expression and Activity. J Biol Chem. 288(6), 4389–4404.
2. Lieve Naesens, Luke Guddat, Dianne Keough, André B.P. van Kuilenburg, Judith Meijer, Johan Vande Voorde and Jan Balzarini (2013). ROLE OF HUMAN HYPOXANTHINE GUANINE 
   PHOSPHORIBOSYLTRANSFERASE IN ACTIVATION 
   OF THE ANTIVIRAL AGENT T-705 (FAVIPIRAVIR). Molecular Pharmacology Fast Forward. 87247,
3. Hicham Bouabe, Klaus Okkenhaug (2013). A Protocol for Construction of Gene Targeting Vectors and Generation of Homologous Recombinant Embryonic Stem Cells. Methods in Molecular Biology. 1064, 337-354.

1. Protocol for Q5® High-Fidelity 2X Master Mix

• Activity of Restriction Enzymes in PCR Buffers
• Guidelines for PCR Optimization with Thermophilic DNA Polymerases

• DNA Polymerase Selection Chart

• PCR Selector
• Tm Calculator

1. What are the advantages of using Q5^® High-Fidelity DNA Polymerase?
   
2. What are the differences between the numerous Q5^® Polymerase products available?
3. My results are not as expected. Where can I find troubleshooting help?
   
4. What ends will my PCR products have?
5. What does exonuclease activity mean for a DNA polymerase?
   
6. What are the properties of this polymerase (fidelity, product ends, max amplicon, modified base incorporation, etc.)?
7. Does Q5^® High-Fidelity DNA Polymerase exhibit a strand displacement activity?
8. How should I determine the appropriate annealing temperature for my reaction?
9. What should the final primer concentration be in my PCR?
10. When and how should I use the Q5^® High GC Enhancer?
11. There is a precipitate in the bottom of the buffer tube. Is this normal?
12. There is a precipitate in the bottom of the Master Mix tube? Is this normal?

• PCR Troubleshooting Guide

• Polymerase Fidelity: What is it, and what does it mean for your PCR?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0492S_L_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0492L_v1_10021856
• M0492L_v1_10032439
• M0492L_v1_10034497
• M0492S_v1_10036749
• M0492S_v1_10044914
• M0492L_v1_10048852
• M0492L_v1_10055342
• M0492S_v1_10060946
• M0492L_v2_10078996
• M0492L_v2_10101237
• M0492L_v2_10112788
• M0492S_v2_10113975
• M0492L_v2_10129013
• M0492S_v2_10129769
• M0492S_v2_10132795
• M0492L_v2_10131653
• M0492L_v2_10147209
• M0492L_v2_10152689
• M0492S_v2_10151806
• M0492S_v2_10157007
• M0492L_v2_10161592
• M0492L_v2_10166259
• M0492L_v2_10170100
• M0492L_v2_10185448
• M0492S_v2_10197194
• M0492S_v2_10211638
• M0492L_v2_10221842
• M0492L_v2_10227791
• M0492L_v2_10232032
• M0492S_v2_10227792
• M0492L_v2_10238402
• M0492L_v2_10246935

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

This product is covered by one or more patents.

This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.

For additional information or to inquire about commercial use, please contact busdev@neb.com.

Trademarks

Q5® is a registered trademark of New England Biolabs, Inc.